Lelios Iva, Greter Melanie
Institute of Experimental Immunology, University of Zürich, Winterthurerstrasse 190, 8057, Zürich, Switzerland.
Methods Mol Biol. 2014;1193:15-9. doi: 10.1007/978-1-4939-1212-4_2.
During neuroinflammatory or neurodegenerative diseases, it is often critical to characterize the composition of infiltrating immune cells. This protocol describes a reliable, fast, and simple method for the isolation of leukocytes from murine central nervous system (CNS) during steady state or inflammation for analysis by flow cytometry or other techniques.
在神经炎症性疾病或神经退行性疾病期间，表征浸润免疫细胞的组成通常至关重要。本方案描述了一种可靠、快速且简单的方法，用于在稳态或炎症期间从小鼠中枢神经系统（CNS）中分离白细胞，以便通过流式细胞术或其他技术进行分析。
