Reißig Sonja, Hackenbruch Christopher, Hövelmeyer Nadine
Institute for Molecular Medicine, University Medical Center, Johannes Gutenberg-University, Obere Zahlbacher Str. 67, 55131, Mainz, Germany,
Methods Mol Biol. 2014;1193:21-5. doi: 10.1007/978-1-4939-1212-4_3.
The lymphocytes of epithelial and lamina proprial compartments of the intestine are phenotypically and functionally distinct and serve a wide range of functions in the intestinal mucosa like regulating intestinal homeostasis, maintaining epithelial barrier function as well as regulating adaptive and innate immune responses. To analyze the role of these cells in different disease states, it is necessary to isolate pure cell populations of the intraepithelial lymphocytes (IEL) and lamina propria lymphocytes (LPL) of the gut. In this protocol we describe a method to isolate T cells from IEL and LPL, which can be used for further investigations like comparative studies of mRNA expression, cell proliferation assay, or protein analysis.
肠道上皮和固有层中的淋巴细胞在表型和功能上存在差异，在肠道黏膜中发挥多种功能，如调节肠道内环境稳定、维持上皮屏障功能以及调节适应性和先天性免疫反应。为了分析这些细胞在不同疾病状态下的作用，有必要分离肠道上皮内淋巴细胞（IEL）和固有层淋巴细胞（LPL）的纯细胞群体。在本方案中，我们描述了一种从IEL和LPL中分离T细胞的方法，该方法可用于进一步的研究，如mRNA表达的比较研究、细胞增殖测定或蛋白质分析。
