Lee Jae-Sung, Oka Kohsuke, Obara Mie, Nishimukai Megumi, Yoo Yung-Choon, Yamada Kaori, Tsukahara Takamitsu, Nakayama Keizo, Hara Hiroshi, Ishizuka Satoshi
Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Biosci Biotechnol Biochem. 2009 Aug;73(8):1732-40. doi: 10.1271/bbb.90035. Epub 2009 Aug 7.
We optimized the isolation protocol for intraepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) from the rat small intestine, and LPLs from even the rat large intestine. The major population of IELs in the small intestine was considered to be from the villus epithelia. The cytotoxicity of mucosal leukocytes was comparable among isolated fractions from both the small and large intestines, regardless of the population differences. Further analyses of the cells collected from other lymphoid tissues demonstrated that CD161(+) cells selectively accumulated in the intestinal lamina propria and did not recirculate through the lymph ducts. Our modified isolation protocol enables the collection of mucosal immune cells from the rat intestines without any deterioration of cell function and could contribute to a better understanding of dietary influences on the mucosal immune system.
我们优化了从大鼠小肠分离上皮内淋巴细胞（IELs）和固有层淋巴细胞（LPLs）以及从大鼠大肠分离LPLs的方案。小肠中IELs的主要群体被认为来自绒毛上皮。无论群体差异如何，小肠和大肠分离组分中黏膜白细胞的细胞毒性相当。对从其他淋巴组织收集的细胞进行的进一步分析表明，CD161(+)细胞选择性地积聚在肠道固有层中，且不会通过淋巴管再循环。我们改进的分离方案能够从大鼠肠道收集黏膜免疫细胞，而不会使细胞功能有任何下降，这有助于更好地理解饮食对黏膜免疫系统的影响。
