一种生成TCR转基因小鼠的方法。
A method for the generation of TCR retrogenic mice.
作者信息
Kieback Elisa, Hilgenberg Ellen, Fillatreau Simon
机构信息
Max Delbrueck Center for Molecular Medicine, Berlin, Germany.
出版信息
Methods Mol Biol. 2014;1193:117-26. doi: 10.1007/978-1-4939-1212-4_12.
PMID:25151002
Abstract
Retrogenic mice provide a unique system for rapidly analyzing the function of genes in the hematopoietic system. Here, we provide a detailed protocol for the production of retrogenic mice expressing genes coding for T cell receptor (TCR) for antigen. This technology should be easy to establish in any laboratory and should allow for a rapid progress in our understanding of the functional roles of TCR repertoires in immunity.
摘要
逆转录基因小鼠为快速分析造血系统中基因的功能提供了一个独特的系统。在此，我们提供了一份详细的方案，用于制备表达编码抗原特异性T细胞受体（TCR）的基因的逆转录基因小鼠。这项技术在任何实验室都应易于建立，并应能使我们在理解TCR库在免疫中的功能作用方面取得快速进展。
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