Zinov'eva V N, Sever I S, Vakhrameeva G M
Mol Gen Mikrobiol Virusol. 1989 Sep(9):33-8.
The plc gene for phospholipase of Pseudomonas aeruginosa, able to be transcribed only from its own promoter, has been introduced into Escherichia coli, Pseudomonas aeruginosa and Pseudomonas putida cells in the recombinant plasmid pPMS21 of a wide host range. The expression of plc gene in all recipient cells has been shown to be phosphate regulated. The fact emphasizes the identity of pho-regulation systems in Escherichia coli and Pseudomonas cells. The level of phospholipase activity is similar in Pseudomonas putida and Pseudomonas aeruginosa under the conditions of the gene derepression, while in Escherichia coli cells the level does not exceed 10% of activity registered in Pseudomonas cells.
铜绿假单胞菌磷脂酶的plc基因只能从其自身启动子转录，已通过广泛宿主范围的重组质粒pPMS21导入大肠杆菌、铜绿假单胞菌和恶臭假单胞菌细胞。已证明plc基因在所有受体细胞中的表达受磷酸盐调节。这一事实强调了大肠杆菌和假单胞菌细胞中pho调节系统的一致性。在基因去阻遏的条件下，恶臭假单胞菌和铜绿假单胞菌中的磷脂酶活性水平相似，而在大肠杆菌细胞中，该水平不超过假单胞菌细胞中记录活性的10%。
