Ali F S, Abdel-Moneim A A
Department of Agric. Microbiol., Fac. Agric., Minia University, Egypt.
Zentralbl Mikrobiol. 1989;144(8):623-8. doi: 10.1016/s0232-4393(89)80126-x.
The effect of 8 growth regulators at concentrations of 1,000, 5,000 and 10,000 ppm on the activity of fungal (Aspergillus flavus var. columnaris) alpha-amylase was studied. Indol acetic acid (IAA) and naphthalene acetic acid (NAA) inhibited alpha-amylase activity by 2% and 7% at 1,000 ppm. The other 6 growth regulators, indol butyric acid (IBA), gibberellic acid, cumarin, cycocel (CCC), atonik-G and kylar, did not inhibit but stimulated alpha-amylase activity (0 to 9%) at 1,000 ppm. All growth regulators studied inhibited alpha-amylase activity at 5,000 and 10,000 ppm concentration except kylar. The effect of organic acids and formaldehyde at 0.01, 0.005, and 0.001 M was studied. Acetic acid stimulated alpha-amylase at all concentrations, but formic acid, oxalic acid, lactic acid and citric acid inhibited alpha-amylase activity by 91, 100, 100 and 79%, respectively, at a concentration of 0.01 M, while by 31, 100, 15 and 20%, respectively, at 0.005 M. Formaldehyde induced 7, 3 and 2% inhibition at 0.01, 0.005 and 0.001 M, respectively. At 0.01 M either sorbitol or fructose inhibited alpha-amylase by 8%, Maltose 7%, sucrose 6%, phenol, glucose and galactose each by 5%, ethanol, glycerol, arabinose and sodium benzoate each by 4%, isopropanol and mannitol 1%, but methanol and ammonium citrate dibasic did not inhibit alpha-amylase. The results indicate that CuCl2, SnCl2, AgNO3 and Fe2(SO4)3 were the strongest inhibitors, followed by Cd(C2H3O2), HgCl2, Na2-EDTA, Na2HPO4, and CaCl2 in decreasing order. NaCl, NaBr and Mn SO4 did not inhibit alpha-amylase at concentrations from 10 mM to 0.01 mM.
研究了8种生长调节剂在1000、5000和10000 ppm浓度下对真菌（黄曲霉柱状变种）α-淀粉酶活性的影响。吲哚乙酸（IAA）和萘乙酸（NAA）在1000 ppm时分别抑制α-淀粉酶活性2%和7%。其他6种生长调节剂，吲哚丁酸（IBA）、赤霉素、香豆素、矮壮素（CCC）、爱多收-G和比久，在1000 ppm时不抑制反而刺激α-淀粉酶活性（0至9%）。除比久外，所有研究的生长调节剂在5000和10000 ppm浓度下均抑制α-淀粉酶活性。研究了0.01、0.005和0.001 M浓度的有机酸和甲醛的影响。乙酸在所有浓度下均刺激α-淀粉酶活性，但甲酸、草酸、乳酸和柠檬酸在0.01 M浓度下分别抑制α-淀粉酶活性91%、100%、100%和79%，而在0.005 M浓度下分别抑制31%、100%、15%和20%。甲醛在0.01、0.005和0.001 M时分别诱导7%、3%和2%的抑制。在0.01 M时，山梨醇或果糖抑制α-淀粉酶8%，麦芽糖抑制7%，蔗糖抑制6%，苯酚、葡萄糖和半乳糖各抑制5%，乙醇、甘油、阿拉伯糖和苯甲酸钠各抑制4%，异丙醇和甘露醇抑制1%，但甲醇和柠檬酸氢二铵不抑制α-淀粉酶。结果表明，CuCl2、SnCl2、AgNO3和Fe2(SO4)3是最强的抑制剂，其次是Cd(C2H3O2)、HgCl2、Na2-EDTA、Na2HPO4和CaCl2，抑制作用依次减弱。NaCl、NaBr和Mn SO4在10 mM至0.01 mM浓度下不抑制α-淀粉酶。
