溶液中功能性肽的表面呈现决定了TAT共轭纳米颗粒的细胞内化效率。
Surface presentation of functional peptides in solution determines cell internalization efficiency of TAT conjugated nanoparticles.
作者信息
Todorova Nevena, Chiappini Ciro, Mager Morgan, Simona Benjamin, Patel Imran I, Stevens Molly M, Yarovsky Irene
机构信息
Health Innovations Research Institute, RMIT University , GPO Box 2476, Melbourne, VIC Australia.
出版信息
Nano Lett. 2014 Sep 10;14(9):5229-37. doi: 10.1021/nl5021848. Epub 2014 Aug 28.
Abstract
Functionalizing nanoparticles with cell-penetrating peptides is a popular choice for cellular delivery. We investigated the effects of TAT peptide concentration and arrangement in solution on functionalized nanoparticles' efficacy for membrane permeation. We found that cell internalization correlates with the positive charge distribution achieved prior to nanoparticle encountering interactions with membrane. We identified a combination of solution based properties required to maximize the internalization efficacy of TAT-functionalized nanoparticles.
摘要
用细胞穿透肽对纳米颗粒进行功能化修饰是细胞递送的常用选择。我们研究了溶液中TAT肽浓度和排列对功能化纳米颗粒膜渗透功效的影响。我们发现细胞内化与纳米颗粒与膜相互作用之前实现的正电荷分布相关。我们确定了使TAT功能化纳米颗粒内化功效最大化所需的基于溶液的性质组合。
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