Spinella S, Liegeard P, Guilbert B, Hontebeyrie-Joskowicz M
Unité d'Immunoparasitologie, Institut Pasteur, Paris, France.
J Autoimmun. 1989 Dec;2(6):791-802. doi: 10.1016/0896-8411(89)90005-x.
Experimental Chagas' disease--infection of mice with the protozoan parasite Trypanosoma cruzi--has been shown to increase the number of Ia-bearing cells in the spleen and the lymph nodes. The majority of these Ia-positive cells were Ig+ and included in the large cell fraction of lymphoid organs from T. cruzi-infected animals indicating that they were activated B cells. These data are consistent with the polyclonal B-cell activation occurring during acute and chronic T. cruzi infection. The levels of secreted natural antibodies, of both IgM and IgG isotypes, were significantly increased in the sera of the infected animals. The present communication demonstrates that in vivo anti-Ia treatment of C3H/HeJ mice infected with the CL strain of T. cruzi suppressed the polyclonal B-cell activation, affecting all the isotypes studied, including IgM, IgG2a and IgG2b, whose levels are predominantly increased during T. cruzi infection. In contrast to the decreased secretion of IgG autoantibodies, the levels of IgM autoantibodies were much less affected. The anti-Ia treatment totally abolished the specific anti-parasite response despite the fact that a pool of Ia-Ig positive cells remained after treatment.
实验性恰加斯病（用原生动物寄生虫克氏锥虫感染小鼠）已被证明会增加脾脏和淋巴结中携带Ia抗原的细胞数量。这些Ia阳性细胞大多数为Ig⁺，并包含在来自克氏锥虫感染动物的淋巴器官的大细胞部分中，这表明它们是活化的B细胞。这些数据与急性和慢性克氏锥虫感染期间发生的多克隆B细胞活化一致。感染动物血清中分泌的天然抗体（IgM和IgG同种型）水平显著升高。本通讯表明，对感染克氏锥虫CL株的C3H/HeJ小鼠进行体内抗Ia治疗可抑制多克隆B细胞活化，影响所研究的所有同种型，包括IgM、IgG2a和IgG2b，其水平在克氏锥虫感染期间主要升高。与IgG自身抗体分泌减少相反，IgM自身抗体水平受影响较小。尽管治疗后仍有一群Ia-Ig阳性细胞存在，但抗Ia治疗完全消除了特异性抗寄生虫反应。
