Seo H, Ando M, Yamauchi K, Matsui N, Takenaka O
Department of Endocrinology and Metabolism, Research Institute of Environmental Medicine, Nagoya, Japan.
Endocrinol Jpn. 1989 Oct;36(5):665-73. doi: 10.1507/endocrj1954.36.665.
Thyroxine(T4)-binding to serum proteins in primates; catarrhini, prosimiae, and platyrrhini were studied by polyacrylamide gel electrophoresis T4 binding analysis. From the electrophoretic analysis, it was shown that thyroxine-binding proteins similar to human thyroxine-binding globulin (TBG) and thyroxine-binding prealbumin (TBPA) were present in catarrhini and prosimiae species, but not in platyrrhini (callithricidae and cebidae). T4-binding analysis also revealed that catarrhini and prosimiae have a high affinity T4-binding protein similar to human TBG. The association constant (Ka) for T4 of the plasma proteins in these species was approximately 2.0 X 10(10) M-1. On the other hand, it was unable to demonstrate a high affinity binding site for T4 in the plasma of platyrrhini species. Both the total and free thyroid hormone concentrations in catarrhini and prosimiae were similar to those in human. Total T4 in cebidae, one of the platyrrhini species, was extremely low. Among 8 animals examined, T4 in 6 was undetectable by radioimmunoassay and the mean T4 of the other two was 2.8 micrograms/dl. However, free thyroid hormone concentrations were similar to those in human. In callithricidae, another platyrrhini species, T4 in plasma was 6.90 +/- 2.11, which is comparable to the level in normal human subjects. However, in this species, high-affinity T4-binding protein was lacking and free thyroid hormone concentrations were extremely high (most were higher than the assay limit). Although the thyroid function of callithricidae remains to be studied, it will be interesting if callithricidae is resistant to thyroid hormone action.
通过聚丙烯酰胺凝胶电泳T4结合分析，研究了灵长类动物（狭鼻猿、原猴亚目和阔鼻猴）甲状腺素（T4）与血清蛋白的结合情况。电泳分析表明，在狭鼻猿和原猴亚目物种中存在与人甲状腺素结合球蛋白（TBG）和甲状腺素结合前白蛋白（TBPA）相似的甲状腺素结合蛋白，但在阔鼻猴（绢毛猴科和卷尾猴科）中不存在。T4结合分析还显示，狭鼻猿和原猴亚目有一种与人TBG相似的高亲和力T4结合蛋白。这些物种血浆蛋白对T4的结合常数（Ka）约为2.0×10¹⁰ M⁻¹。另一方面，在阔鼻猴物种的血浆中未能证明存在T4的高亲和力结合位点。狭鼻猿和原猴亚目的总甲状腺激素浓度和游离甲状腺激素浓度与人类相似。阔鼻猴物种之一卷尾猴科的总T4极低。在检查的8只动物中，6只的T4通过放射免疫测定法无法检测到，另外两只的平均T4为2.8微克/分升。然而，游离甲状腺激素浓度与人类相似。在阔鼻猴的另一个物种绢毛猴科中，血浆中的T4为6.90±2.11，与正常人类受试者的水平相当。然而，在这个物种中，缺乏高亲和力T4结合蛋白，游离甲状腺激素浓度极高（大多数高于检测限）。尽管绢毛猴科的甲状腺功能仍有待研究，但如果绢毛猴科对甲状腺激素作用有抗性将很有趣。
