Tadayoni Mehrnoosh, Sheikh-Zeinoddin Mahmoud, Soleimanian-Zad Sabihe
Department of Food Science, College of Agriculture, Isfahan University of Technology, Isfahan, Iran, Islamic Azad University Baghmalek Branch, Baghmalek, Iran.
Department of Food Science, College of Agriculture, Isfahan University of Technology, Isfahan, Iran.
Int J Biol Macromol. 2015 Jan;72:179-84. doi: 10.1016/j.ijbiomac.2014.08.015. Epub 2014 Aug 23.
The aim of this study was to evaluate the prebiotic potential and some functional properties of polysaccharides isolated from acorn fruit. The FTIR spectrum of isolated acorn polysaccharide (IAP) showed the typical bands corresponding to sugars and polysaccharides. The IAP was resistant to simulated acidic and enzymatic digestion even more than Inulin (In). The prebiotic activity, which was tested using IAP as a carbon source, showed significant increase in the growth and viability of Lactobacillus plantarum A7 (probiotic). Viability of Lactobacillus plantarum A7 in IAP and In supplemented media was stable even after 72 h, whereas in glucose supplemented medium, bacterial growth showed a notable decrease after 24h. Lipid absorption capacity (LAC) and water holding capacity (WHC) of IAP were 5.44 ± 0.02 (g oil/g DM) and 4.33 ± 0.03 (g water/g DM), respectively, which were comparable to some dietary fibers and were more than In. IAP scavenged DPPH radicals by 82.24%. IAP was found to have a high scavenging ability compared to the reference prebiotic (In), giving a scavenging ability of about 20%. Therefore, due to prebiotic capability, high WHC, LAC and good antioxidant activity, IAP can be a suitable candidate for technological applications and health improving effects in functional food.
本研究的目的是评估从橡子果实中分离出的多糖的益生元潜力和一些功能特性。分离出的橡子多糖（IAP）的傅里叶变换红外光谱显示出与糖类和多糖相对应的典型谱带。IAP对模拟酸性和酶消化具有抗性，甚至比菊粉（In）更甚。以IAP作为碳源测试的益生元活性表明，植物乳杆菌A7（益生菌）的生长和活力显著增加。即使在72小时后，在添加IAP和In的培养基中植物乳杆菌A7的活力仍保持稳定，而在添加葡萄糖的培养基中，24小时后细菌生长显著下降。IAP的脂质吸收能力（LAC）和持水能力（WHC）分别为5.44±0.02（g油/g干物质）和4.33±0.03（g水/g干物质），与一些膳食纤维相当且高于In。IAP对DPPH自由基的清除率为82.24%。与参考益生元（In）相比，IAP具有较高的清除能力，清除能力约为20%。因此，由于其益生元能力、高WHC、LAC和良好的抗氧化活性，IAP可成为功能性食品技术应用和改善健康效果的合适候选物。
