寻找增强子-启动子相互作用特异性的决定因素。
In search of the determinants of enhancer-promoter interaction specificity.
作者信息
van Arensbergen Joris, van Steensel Bas, Bussemaker Harmen J
机构信息
Division of Gene Regulation, Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands.
Division of Gene Regulation, Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands.
出版信息
Trends Cell Biol. 2014 Nov;24(11):695-702. doi: 10.1016/j.tcb.2014.07.004. Epub 2014 Aug 24.
Abstract
Although it was originally believed that enhancers activate only the nearest promoter, recent global analyses enabled by high-throughput technology suggest that the network of enhancer-promoter interactions is far more complex. The mechanisms that determine the specificity of enhancer-promoter interactions are still poorly understood, but they are thought to include biochemical compatibility, constraints imposed by the three-dimensional architecture of chromosomes, insulator elements, and possibly the effects of local chromatin composition. In this review, we assess the current insights into these determinants, and highlight the functional genomic approaches that will lead the way towards better mechanistic understanding.
摘要
虽然最初人们认为增强子仅激活最近的启动子，但最近高通量技术实现的全局分析表明，增强子与启动子相互作用的网络要复杂得多。决定增强子与启动子相互作用特异性的机制仍知之甚少，但人们认为这些机制包括生化兼容性、染色体三维结构施加的限制、绝缘子元件以及可能的局部染色质组成的影响。在这篇综述中，我们评估了目前对这些决定因素的认识，并强调了将引领我们更好地理解其机制的功能基因组学方法。
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