Morosini P P, Sarzani R, Arnaldi G, Taccaliti A
Minerva Endocrinol. 1989 Jul-Sep;14(3):153-8.
In 12 patients with hypothalamic amenorrhea and in 5 normal women, plasma gonadotropins (LH and FSH) were assayed before and 20, 30, 60, and 120 minutes after stimulation with 10 mcg GnRH i.v. and 24 hours after stimulation with 100 mcg GnRH i.v. In four patients and in controls the pulsatile secretion of LH was evaluated by blood sampling at 15 minute intervals for the 24-hour period. All patients showed different increases in LH after administration of 10 mcg and 100 mcg at 60 and 120 minutes. In two patients, with decreased LH pulse frequency, the gonadotropin increase is dose-dependent in respect to GnRH. In the other two, with normal LH pulse frequency, no difference was shown. In conclusion, this study suggests that the mechanism responsible for amenorrhea is due to reduced frequency of pulsatile GnRH secretion. However, in some patients LH pulse frequency was within the normal range. The double GnRH test (10 or 100 mcg) may be useful in distinguishing these different forms of amenorrhea.
对12例下丘脑性闭经患者和5名正常女性，在静脉注射10微克促性腺激素释放激素（GnRH）前以及注射后20、30、60和120分钟，以及静脉注射100微克GnRH后24小时，测定血浆促性腺激素（促黄体生成素和促卵泡生成素）。对4例患者和对照组，通过在24小时内每隔15分钟采血来评估促黄体生成素的脉冲式分泌。所有患者在注射10微克和100微克GnRH后60和120分钟时促黄体生成素均有不同程度升高。在2例促黄体生成素脉冲频率降低的患者中，促性腺激素升高与GnRH呈剂量依赖性。在另外2例促黄体生成素脉冲频率正常的患者中，未显示出差异。总之，本研究提示闭经的机制是促性腺激素释放激素脉冲式分泌频率降低。然而，部分患者促黄体生成素脉冲频率在正常范围内。双重GnRH试验（10或100微克）可能有助于区分这些不同类型的闭经。
