Sawoo Olivier, Dublineau Amélie, Batéjat Christophe, Zhou Paul, Manuguerra Jean-Claude, Leclercq India
Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France; University of Paris Diderot, Sorbonne Paris Cité (Cellule Pasteur), Paris, France.
Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France.
PLoS One. 2014 Aug 28;9(8):e106192. doi: 10.1371/journal.pone.0106192. eCollection 2014.
Influenza A viruses (IAVs) are a major cause of infectious respiratory human diseases and their transmission is dependent upon the environment. However, the role of environmental factors on IAV survival outside the host still raises many questions. In this study, we used lentiviral pseudotypes to study the influence of the hemagglutinin protein in IAV survival. High-titered and cleaved influenza-based lentiviral pseudoparticles, through the use of a combination of two proteases (HAT and TMPRSS2) were produced. Pseudoparticles bearing hemagglutinin proteins derived from different H1N1, H3N2 and H5N1 IAV strains were subjected to various environmental parameters over time and tested for viability through single-cycle infectivity assays. We showed that pseudotypes with different HAs have different persistence profiles in water as previously shown with IAVs. Our results also showed that pseudotypes derived from H1N1 pandemic virus survived longer than those derived from seasonal H1N1 virus from 1999, at high temperature and salinity, as previously shown with their viral counterparts. Similarly, increasing temperature and salinity had a negative effect on the survival of the H3N2 and H5N1 pseudotypes. These results showed that pseudotypes with the same lentiviral core, but which differ in their surface glycoproteins, survived differently outside the host, suggesting a role for the HA in virus stability.
甲型流感病毒（IAVs）是人类呼吸道感染性疾病的主要病因，其传播取决于环境。然而，环境因素对IAV在宿主体外存活的作用仍存在许多疑问。在本研究中，我们使用慢病毒假型来研究血凝素蛋白对IAV存活的影响。通过使用两种蛋白酶（HAT和TMPRSS2）的组合，制备了高滴度且经裂解的基于流感病毒的慢病毒假颗粒。携带源自不同H1N1、H3N2和H5N1 IAV毒株的血凝素蛋白的假颗粒随时间接受各种环境参数处理，并通过单循环感染性试验检测其活力。我们发现，与之前对IAV的研究结果一样，具有不同血凝素的假型在水中具有不同的持久性特征。我们的结果还表明，源自甲型H1N1大流行病毒的假型在高温和高盐度条件下比源自1999年季节性H1N1病毒的假型存活时间更长，这与它们的病毒对应物的情况一致。同样，温度和盐度的升高对H3N2和H5N1假型的存活产生负面影响。这些结果表明，具有相同慢病毒核心但表面糖蛋白不同的假型在宿主体外的存活情况不同，这表明血凝素在病毒稳定性中发挥了作用。
