中国南方壮族人群中MIC基因多态性与单倍型多样性

MIC gene polymorphism and haplotype diversity in Zhuang nationality of Southern China.

作者信息

Chen E, Lin L, Chen C J, Zhang X Y, Luo Q Z, Yu P

机构信息

Department of Immunology, College of Basic Medical Sciences, Central South University, 110 Xiangya Road, Changsha 410078, China.

Department of Microbiology, Changzhi Medical College, Changzhi 046000, China.

出版信息

Hum Immunol. 2014 Sep;75(9):953-9. doi: 10.1016/j.humimm.2014.08.203. Epub 2014 Aug 27.

DOI:10.1016/j.humimm.2014.08.203

PMID:25167773

Abstract

Zhuang ethnic minority is the largest minority group in China. Here, we report for the first time the polymorphisms of MICA and MICB in a healthy Zhuang population of 209 unrelated individuals. Using polymerase chain reaction-sequence specific priming (PCR-SSP) and sequencing-based typing (PCR-SBT), 13 MICA-sequence alleles and 5 MICA-STR alleles, as well as 11 MICB alleles were detected, among which MICA(∗)010, MICA(∗)A5 and MICB(∗)005:02 were the most frequent alleles. Linkage disequilibria was investigated and the most common two-locus haplotypes were MICB(∗)005:02-MICA(∗)010 and MICB(∗)014-MICA(∗)045. These results suggest informative genetic markers for investigating origins and evolution of MHC class I region haplotypes in Zhuang population.

摘要

壮族是中国最大的少数民族。在此，我们首次报告了209名无亲缘关系的健康壮族人群中MICA和MICB的多态性。使用聚合酶链反应-序列特异性引物（PCR-SSP）和基于测序的分型（PCR-SBT），检测到13个MICA序列等位基因、5个MICA-STR等位基因以及11个MICB等位基因，其中MICA(∗)010、MICA(∗)A5和MICB(∗)005:02是最常见的等位基因。研究了连锁不平衡，最常见的两位点单倍型是MICB(∗)005:02-MICA(∗)010和MICB(∗)014-MICA(∗)045。这些结果为研究壮族人群中MHC I类区域单倍型的起源和进化提供了有用的遗传标记。