中国南方黎族MIC基因多态性与单倍型多样性
MIC gene polymorphism and haplotype diversity in Li nationality of Southern China.
作者信息
Lin L, Yang W, Chen E, Gong Z, Luo Q Z, Wei X B, Yu P
机构信息
Department of Immunology, College of Basic Medical Sciences, Central South University, Changsha, 410078, China.
出版信息
Tissue Antigens. 2015 Jan;85(1):45-9. doi: 10.1111/tan.12449. Epub 2014 Dec 2.
Here, we report for the first time the polymorphisms of MICA and MICB in a healthy Li population of 344 unrelated individuals. By using polymerase chain reaction-sequence specific priming (PCR-SSP) and sequence-based typing (PCR-SBT), 17 MICA-sequence alleles and 5 MICA-STR (short tandem repeats, STR) alleles, as well as 17 MICB alleles were detected, among which MICA010, MICAA4 and MICB005:02 were the most frequent alleles. In addition, linkage disequilibrium was investigated and the most common two-locus haplotypes were MICB005:02-MICA010 and MICB008-MICA*002:01. These results present informative genetic markers for the investigation of possible origins and the evolution of MHC class I haplotypes in the Li population.
在此，我们首次报告了344名无亲缘关系的健康黎族个体中MICA和MICB的多态性。通过聚合酶链反应-序列特异性引物法（PCR-SSP）和基于序列的分型法（PCR-SBT），检测到17个MICA序列等位基因、5个MICA短串联重复序列（STR）等位基因以及17个MICB等位基因，其中MICA010、MICAA4和MICB005:02是最常见的等位基因。此外，还研究了连锁不平衡，最常见的两位点单倍型是MICB005:02-MICA010和MICB008-MICA*002:01。这些结果为研究黎族群体中MHC I类单倍型的可能起源和进化提供了有用的遗传标记。
Zotero 插件