Serasanambati Mamatha, Chilakapati Shanmuga Reddy, Manikonda Pavan Kumar, Kanala Jagadeeswara Reddy, Chilakapati Damodar Reddy
a Department of Biochemistry , S. V. University , Sugen Life Sciences Pvt Ltd, #4/86, S.V. Nagar, Perumalla Palli, Tirupati 517505 , Andhra Pradesh , India.
Nat Prod Res. 2015;29(5):484-90. doi: 10.1080/14786419.2014.951932. Epub 2014 Aug 28.
This study was designed to investigate the combination effects of brucine and gemcitabine, each with anticancer properties, in MCF-7 human breast cancer cells in culture. With regard to cell viability, effects of both the drugs and their combinations were inversely proportional to dose and time. For various proportional drug combinations studied, combination effects were analysed using CompuSyn software. The analyses revealed synergistic and/or additive effects regarding cell viability, anchorage-independent growth and cell migration. Combination analyses exhibited diversified impacts of the type of combination treatment, namely pretreatment with either drug followed by exposure to the other, or treatment with both drugs at the same time. Compared with untreated cells, combination treatment of asynchronised MCF-7 cells resulted in 17.2 × decrease in G2 phase, increasing G1 (2.1 × ) and S (1.5 × ) phase cells in cell cycle analysis. Brucine, either individually or in combination, but not gemcitabine, inhibited NF-kB subunit (p65) expression in MCF-7 cells.
本研究旨在探讨具有抗癌特性的马钱子碱和吉西他滨在培养的MCF-7人乳腺癌细胞中的联合效应。关于细胞活力，两种药物及其组合的效应与剂量和时间成反比。对于所研究的各种比例的药物组合，使用CompuSyn软件分析联合效应。分析显示，在细胞活力、非锚定依赖性生长和细胞迁移方面存在协同和/或相加效应。联合分析显示联合治疗类型具有多样化的影响，即先用一种药物预处理，然后再接触另一种药物，或同时使用两种药物进行治疗。与未处理的细胞相比，异步MCF-7细胞的联合治疗导致细胞周期分析中G2期细胞减少17.2倍，G1期（2.1倍）和S期（1.5倍）细胞增加。马钱子碱单独或联合使用均可抑制MCF-7细胞中NF-κB亚基（p65）的表达，而吉西他滨则无此作用。
