Souto-Padrón T, Reyes M B, Leguizamon S, Campetella O E, Frasch A C, de Souza W
Laboratório de Ultraestrutura Celular e Microscopia Eletrônica, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
Eur J Cell Biol. 1989 Dec;50(2):272-8.
Polyclonal antibodies obtained against antigenic proteins encoded by six recombinant DNA clones of Trypanosoma cruzi were used for the ultrastructural localization of the respective antigens in thin sections of parasites (epimastigote, amastigote and trypomastigote forms of T. cruzi) embedded at low temperature in Lowicryl K4M resin. Antigens of high molecular weight containing tandemly repeated amino acid sequence motifs and recognized by sera from patients with Chagas' disease, were located only in the flagellum, where it contacts the parasite cell body. Other antigens were located on the surface of the parasite while still others were found within the flagellar pocket, as is the case with an antigen released during the acute phase of Chagas' disease. Thus, we conclude that some of the T. cruzi proteins which are antigenic in human infections are located in defined regions of the parasite. Some of the antigens were not expressed to the same extent in the three different developmental stages of the parasite.
针对克氏锥虫六个重组DNA克隆所编码的抗原蛋白获得的多克隆抗体，用于在低温下包埋于Lowicryl K4M树脂中的寄生虫（克氏锥虫的上鞭毛体、无鞭毛体和锥鞭毛体形式）薄切片中各自抗原的超微结构定位。含有串联重复氨基酸序列基序且被恰加斯病患者血清识别的高分子量抗原，仅位于鞭毛与寄生虫细胞体接触的部位。其他抗原位于寄生虫表面，还有一些抗原存在于鞭毛袋内，恰加斯病急性期释放的一种抗原就是如此。因此，我们得出结论，在人类感染中具有抗原性的一些克氏锥虫蛋白位于寄生虫的特定区域。其中一些抗原在寄生虫的三个不同发育阶段表达程度不同。
