白色念珠菌和光滑念珠菌生物膜在中间和成熟发育阶段对银纳米颗粒的敏感性。

Susceptibility of Candida albicans and Candida glabrata biofilms to silver nanoparticles in intermediate and mature development phases.

作者信息

Monteiro Douglas Roberto, Takamiya Aline Satie, Feresin Leonardo Perina, Gorup Luiz Fernando, de Camargo Emerson Rodrigues, Delbem Alberto Carlos Botazzo, Henriques Mariana, Barbosa Debora Barros

机构信息

Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Univ Estadual Paulista (UNESP), 16015-050 Araçatuba, São Paulo, Brazil.

出版信息

J Prosthodont Res. 2015 Jan;59(1):42-8. doi: 10.1016/j.jpor.2014.07.004. Epub 2014 Aug 26.

DOI:10.1016/j.jpor.2014.07.004

PMID:25168655

Abstract

PURPOSE

The aim of this study was to investigate the susceptibility of Candida albicans and Candida glabrata biofilm development, in their intermediate and maturation stages, to the influence of silver nanoparticles (SN).

METHODS

SN (5 nm) suspensions were synthesized via reduction of silver nitrate by a solution of sodium citrate. These suspensions were used to treat Candida biofilms for five hours, grown on acrylic surfaces for 24-h (intermediate stage) and 48-h (maturation stage), and their efficacy was determined by total biomass (using crystal violet staining) and colony forming units (CFUs) quantification.

RESULTS

SN promoted significant reductions (p<0.05) in the total biomass and number of CFUs of Candida biofilms, ranging from 23% to 51.5% and 0.63 to 1.59-log10, respectively. Moreover, there were no significant differences in the total biofilm biomass (p>0.05), when the different stages of biofilm development (24 or 48h) were exposed to SN. Comparing the number of CFUs between 24- and 48-h biofilms treated with SN, a significant difference (p<0.05) was found only for the C. albicans 324LA/94 strain.

CONCLUSIONS

In general, the intermediate and maturation stages of biofilm development do not interfere in the susceptibility of C. albicans and C. glabrata biofilms to SN. These findings are fundamental for the deployment of new therapies aimed at preventing denture stomatitis.

摘要

目的

本研究旨在调查白色念珠菌和光滑念珠菌生物膜在其形成中期和成熟阶段对银纳米颗粒（SN）影响的敏感性。

方法

通过柠檬酸钠溶液还原硝酸银合成了5纳米的SN悬浮液。这些悬浮液用于处理在丙烯酸表面生长24小时（中期）和48小时（成熟阶段）的念珠菌生物膜5小时，其效果通过总生物量（使用结晶紫染色）和菌落形成单位（CFU）定量来确定。

结果

SN使念珠菌生物膜的总生物量和CFU数量显著减少（p<0.05），分别从23%降至51.5%和0.63至1.59个对数10。此外，当生物膜发育的不同阶段（24或48小时）暴露于SN时，总生物膜生物量没有显著差异（p>0.05）。比较用SN处理的24小时和48小时生物膜之间的CFU数量，仅在白色念珠菌324LA/94菌株中发现显著差异（p<0.05）。