Dorow D S, Burke J, Goding J W
Monash University Department of Pathology and Immunology, Vic.
Aust N Z J Med. 1989 Dec;19(6):677-81. doi: 10.1111/j.1445-5994.1989.tb00332.x.
In 1986 Ordovas et al., reported that a polymorphism in the 3' flanking region of the apolipoprotein AI gene was strongly associated with premature coronary artery disease. This polymorphism affects a restriction site for the endonuclease PstI, resulting in the identification of a 3.3 kb band, rather than the more common 2.2 kb band, when genomic blots of PstI digested human DNA are probed with an apolipoprotein AI gene probe. In a study population of 88 patients with severe coronary artery disease before the age of 60, 28 (32%) carried the 3.3 kb allele, which was found in only five (4%) of 123 randomly chosen control subjects. In the present study, we have assessed the prevalence of this polymorphism in coronary artery disease patients and outpatients with abnormal lipid levels at the Alfred Hospital, Melbourne, and in normal volunteers. The 3.3 kb allele was present in 7-12% of subjects in these populations, and showed no association with coronary artery disease.
1986年，奥尔多维瓦斯等人报告称，载脂蛋白AI基因3'侧翼区域的多态性与早发性冠状动脉疾病密切相关。这种多态性影响了核酸内切酶PstI的一个限制性位点，当用载脂蛋白AI基因探针探测经PstI消化的人类DNA的基因组印迹时，会识别出一条3.3 kb的条带，而不是更常见的2.2 kb条带。在一项针对88名60岁前患有严重冠状动脉疾病的患者的研究群体中，28名（32%）携带3.3 kb等位基因，而在123名随机选择的对照受试者中，只有5名（4%）携带该等位基因。在本研究中，我们评估了墨尔本阿尔弗雷德医院的冠状动脉疾病患者、血脂水平异常的门诊患者以及正常志愿者中这种多态性的患病率。在这些人群中，7%至12%的受试者存在3.3 kb等位基因，且该等位基因与冠状动脉疾病无关联。
