Dyszkiewicz W, Flameng W, Minten J
Mater Med Pol. 1989 Jan-Mar;21(1):15-9.
In 12 dogs the hearts after excision were perfused for 24 hours with Bretschneider HTK cardioplegic solution. Six of these hearts were used only to assess myocardial HEP and ultrastructure during 24 hours of conservation. In the next six dogs orthotopic heart transplantation was performed to evaluate functional outcome after prolonged preservation. After 24 hours of continuous perfusion of the donor heart the ATP level was completely comparable with control, preischemic value. Also ultrastructure of the myocytes was perfectly preserved. All transplanted hearts recovered completely upon reperfusion without a need of inotropic support. Good functional outcome after transplantation was correlated with about 70% of myocardial HEP content and intact ultrastructure of the myocytes. We concluded that continuous perfusion with Bretschneider HTK cardioplegic solution makes successful heart transplantation possible after 24 hours of preservation.
在12只犬中，切除后的心脏用布雷施奈德HTK心脏停搏液灌注24小时。其中6只心脏仅用于评估保存24小时期间的心肌高能磷酸化合物（HEP）和超微结构。在另外6只犬中进行了原位心脏移植，以评估长时间保存后的功能结果。供体心脏连续灌注24小时后，ATP水平与缺血前对照值完全相当。心肌细胞的超微结构也保存完好。所有移植心脏再灌注后完全恢复，无需使用正性肌力药物支持。移植后良好的功能结果与约70%的心肌HEP含量以及心肌细胞完整的超微结构相关。我们得出结论，用布雷施奈德HTK心脏停搏液进行连续灌注使得在保存24小时后成功进行心脏移植成为可能。
