不同细胞类型中YB-1核分布的免疫细胞化学研究

Immunocytochemical study of YB-1 nuclear distribution in different cell types.

作者信息

Bogolyubova I O, Lyabin D N, Bogolyubov D S, Ovchinnikov L P

机构信息

Laboratory of Cell Morphology, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Avenue, 194064 St. Petersburg, Russia.

Institute of Protein Research, Russian Academy of Sciences, 4 Institutskaya Street, 142290 Pushchino, Moscow Region, Russia.

出版信息

Tissue Cell. 2014 Dec;46(6):457-61. doi: 10.1016/j.tice.2014.08.002. Epub 2014 Aug 13.

DOI:10.1016/j.tice.2014.08.002

PMID:25175035

Abstract

In the present work we studied the distribution of YB-1 in the nuclei of mouse hepatocytes, early embryos and human skin fibroblasts with the use of light and electron microscopy. To reveal YB-1, we applied rat polyclonal antibody against the C-terminal fragment of YB-1 molecule and rabbit polyclonal antibody against full-length YB-1 molecule. YB-1 distribution patterns varied significantly in different cell types. YB-1 was found to be colocalized with RNA polymerase I in mouse hepatocytes and embryos. Besides, YB-1 was revealed in a population of Cajal bodies in 2-cell mouse embryos but not in other cells studied.

摘要

在本研究中，我们利用光学显微镜和电子显微镜研究了YB-1在小鼠肝细胞、早期胚胎和人类皮肤成纤维细胞核中的分布。为了检测YB-1，我们应用了针对YB-1分子C末端片段的大鼠多克隆抗体和针对全长YB-1分子的兔多克隆抗体。YB-1的分布模式在不同细胞类型中差异显著。在小鼠肝细胞和胚胎中，发现YB-1与RNA聚合酶I共定位。此外，在2细胞期小鼠胚胎的一群 Cajal 体中发现了YB-1，但在其他研究的细胞中未发现。