Machado T L, Formiga L C, Machado R D, da Silva R R
Instituto de Microbiologia, UFRJ, Rio de Janeiro, Brasil.
Mem Inst Oswaldo Cruz. 1989 Apr-Jun;84(2):241-3. doi: 10.1590/s0074-02761989000200013.
The isolation of toxigenic Corynebacterium diphtheriae from sperm is reported. The organism was identified through the investigation of fluorescence under the UV light, the presence of pirazinecarboxilamidase enzyme (Pyz), in vitro and in vivo and virulence methods (single radial immunodiffusion, cell culture, guinea pig intradermic test). The strain was initially considered nontoxinogenic by single radial immunodiffusion, but its virulence was observed afterwards, when we applied the tests already mentioned. The strain could be considered a "Diphtheroid" without adequate specification.
有报告称从精液中分离出产毒白喉棒状杆菌。通过在紫外光下观察荧光、检测吡嗪羧酰胺酶(Pyz)的存在、采用体外和体内毒力检测方法(单向辐射免疫扩散、细胞培养、豚鼠皮内试验)对该微生物进行了鉴定。该菌株最初通过单向辐射免疫扩散被认为不产毒素,但在我们应用上述检测方法后,观察到了它的毒力。该菌株在没有充分特征描述的情况下可被视为一种“类白喉杆菌”。