Xu Zhiyang, Jiang Guoying, Lin Shiqing, Guan Jun, Chen Guodu, Chen Guanze
Department of Surgery, Putian First Hospital, Teaching Hospital of Fujian Med University, Putian 351100, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Jul;34(8):1171-5.
To observe the effect of N-acetylcysteine (NAC) on intestine injury induced by cardiopulmonary bypass (CPB) in rats.
Thirty-two rats were randomly divided into sham-operated group, NAC control group, CPB model group, and CPB plus NAC treatment group (n=8). In the latter two groups, the rats were subjected to CPB for 1 h. The rats received intraperitoneal injections of normal saline or NAC (0.5 g/kg) as appropriate for 3 successive days prior to CPB, and those in CPB plus NAC group were given NAC (100 mg/kg) in CPB prime followed by infusion at 20 mgsol;(kg·h) until the cessation of CPB. Intestinal and blood samples were collected 2 h after CPB for pathological analysis and measurement of intestinal concentrations of malondialdehyde (MDA), tumor necrosis factor (TNF)-α, interlukin (IL)-6 and activity of superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px) and serum levels of diamine oxidase (DAO).
Evident oxidative stress and pathological damages of the intestines were observed in rats after CPB. NAC treatment obviously alleviated intestinal damages induced by CPB, decreased the levels of intestinal MDA, TNF-α, IL-6 and serum DAO and increased activity of SOD, GSH, and GSH-Px in the intestines.
Perioperative NAC treatment can alleviate intestinal injury induced by CPB in rats by suppressing oxidative stress and inflammatory response.
观察N-乙酰半胱氨酸（NAC）对大鼠体外循环（CPB）所致肠损伤的影响。
32只大鼠随机分为假手术组、NAC对照组、CPB模型组和CPB + NAC治疗组（n = 8）。后两组大鼠进行1小时的CPB。在CPB前连续3天，大鼠分别腹腔注射适量生理盐水或NAC（0.5 g/kg），CPB + NAC组大鼠在CPB预充液中加入NAC（100 mg/kg），随后以20 mg/（kg·h）持续输注直至CPB结束。CPB后2小时采集肠组织和血液样本，进行病理分析，并检测肠组织中丙二醛（MDA）、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6的浓度以及超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）的活性，同时检测血清二胺氧化酶（DAO）水平。
CPB后大鼠出现明显的氧化应激和肠道病理损伤。NAC治疗明显减轻了CPB所致的肠道损伤，降低了肠组织中MDA、TNF-α、IL-6的水平以及血清DAO水平，并提高了肠组织中SOD、GSH、GSH-Px的活性。
围手术期NAC治疗可通过抑制氧化应激和炎症反应减轻大鼠CPB所致的肠损伤。
