一种新型免疫层析试验在检测母体和脐带血清中幽门螺杆菌抗体的应用。

The utilization of a new immunochromatographic test in detection of Helicobacter pylori antibody from maternal and umbilical cord serum.

作者信息

Kuo Fu-Chen, Wu Chien-Yi, Kuo Chao-Hung, Wu Chia-Fang, Lu Chien-Yu, Chen Yen-Hsu, Chen Chiao-Yun, Lo Yi-Ching, Wu Ming-Tsang, Hu Huang-Ming

机构信息

Graduate Institute of Public Health, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; Department of Gynecology and Obstetrics, E-Da Hospital, Kaohsiung 824, Taiwan ; School of Medicine, College of Medicine, I-Shou University, Kaohsiung 824, Taiwan.

Department of Pediatrics, E-Da Hospital, I-Shou University, Kaohsiung 824, Taiwan.

出版信息

Biomed Res Int. 2014;2014:568410. doi: 10.1155/2014/568410. Epub 2014 Aug 7.

DOI:10.1155/2014/568410

PMID:25177695

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4142160/

Abstract

BACKGROUND

Helicobacter pylori (H. pylori) was linked with several extragastrointestinal diseases, including preeclampsia and intrauterine growth restriction of fetus. One of the signals which can be transferred from mother to fetus is the H. pylori IgG antibody. Aims. We utilized a commercial immunochromatographic kit to detect the antibody in maternal and cord serum.

METHODS

Three hundred and forty-six females were enrolled and the blood samples were collected on antenatal examination and on delivery. The maternal H. pylori infection was determined by stool H. pylori antigen test.

RESULTS

One hundred and five females (30.3%) were H. pylori-infected, and the prevalence was higher in immigrants (43.5%) than in Taiwanese (28.7%, P = 0.058). The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the kit were 77.1%, 88.0%, 73.6%, 89.8%, and 84.7%, respectively. This kit also had similar performance in cord serum. Comparing to the maternal result on delivery, this kit offered a consistent performance in antenatal maternal serum (kappa coefficient 0.92) and in cord serum (kappa coefficient 0.88).

CONCLUSIONS

H. pylori IgG antibody can be transferred through the placenta into the fetal circulation. However, accuracy of the test kit needs to be evaluated before utilization in screening.

摘要

背景

幽门螺杆菌（H. pylori）与多种胃肠道外疾病有关，包括先兆子痫和胎儿宫内生长受限。幽门螺杆菌IgG抗体是可以从母亲传递给胎儿的信号之一。目的：我们使用一种商用免疫层析试剂盒检测母体和脐带血清中的抗体。

方法

招募了346名女性，并在产前检查和分娩时采集血样。通过粪便幽门螺杆菌抗原检测确定母体幽门螺杆菌感染情况。

结果

105名女性（30.3%）感染了幽门螺杆菌，移民中的患病率（43.5%）高于台湾女性（28.7%，P = 0.058）。该试剂盒的敏感性、特异性、阳性预测值、阴性预测值和准确性分别为77.1%、88.0%、73.6%、89.8%和84.7%。该试剂盒在脐带血清中的表现也相似。与分娩时的母体结果相比，该试剂盒在产前母体血清（kappa系数0.92）和脐带血清（kappa系数0.88）中的表现一致。