超临界流体萃取作为干血斑质谱分析的一种制备方法。
Supercritical fluid extraction as a preparation method for mass spectrometry of dried blood spots.
作者信息
Matsubara Atsuki, Izumi Yoshihiro, Nishiumi Shin, Suzuki Makoto, Azuma Takeshi, Fukusaki Eiichiro, Bamba Takeshi, Yoshida Masaru
机构信息
Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan.
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Oct 15;969:199-204. doi: 10.1016/j.jchromb.2014.08.013. Epub 2014 Aug 17.
The potential of supercritical fluid extraction (SFE) as a preparation method for mass spectrometry of dried blood spots (DBS) was examined. SFE is generally used for the extraction of hydrophobic compounds, but hydrophilic metabolites such as amino acids, amines, and nucleic-acid-related metabolites could be extracted by adding a low level of methanol as a modifier. Under the optimized conditions, over 200 metabolites were detected from a dried serum spot, of which over 160 metabolites could be analyzed stably (RSD <20%). These results show that SFE is an effective extraction method of metabolites with a wide range of polarity in DBS.
考察了超临界流体萃取（SFE）作为干血斑（DBS）质谱分析样品制备方法的潜力。SFE通常用于萃取疏水性化合物，但通过添加低水平的甲醇作为改性剂，可以萃取亲水性代谢物，如氨基酸、胺类和核酸相关代谢物。在优化条件下，从干血清斑中检测到200多种代谢物，其中160多种代谢物能够被稳定分析（相对标准偏差<20%）。这些结果表明，SFE是一种有效萃取DBS中具有广泛极性代谢物的方法。
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