Valleh Mehdi Vafaye, Tahmoorespur Mojtaba, Joupari Morteza Daliri, Dehghani Hesam, Rasmussen Mikkel Aabech, Hyttel Poul, Strøbech Lotte
Department of Animal Science,Faculty of Agriculture,University of Zabol,P.O. Box 98615-538,Zabol,Iran.
Department of Animal Science,Faculty of Agriculture,Ferdowsi University of Mashhad,Mashhad,I.R.Iran.
Zygote. 2015 Oct;23(5):712-21. doi: 10.1017/S0967199414000367. Epub 2014 Sep 2.
The effects of the paternal breed on early embryo and later pre- and postnatal development are well documented. Several recent studies have suggested that such paternal effects may be mediated by the paternally induced epigenetic modifications during early embryogenesis. The objective of this study was to investigate the effects of the paternal breed on the early embryonic development and relative expression of the maternally imprinted gene, IGF-II, and the apoptosis-related genes BAK1 and BCL2-L1 in in vitro produced (IVP) bovine embryos derived from two unrelated paternal breeds (Holstein and Brown Swiss). The degree of correlation of IGF-II expression pattern with embryo developmental competence and apoptosis-related genes was also investigated. The relative abundance of IGF-II, BCL2-L1 and BAK1 transcripts in day 8 embryos was measured by quantitative reverse-transcription polymerase chain reaction using the comparative Cp method. Our data revealed that the paternal breed did not influence cleavage rate, blastocyst rate and relative abundance of IGF-II, BAK1 and BCL2-L1 in day 8 blastocysts (P > 0.05). Nevertheless, IGF-II expression levels were highly correlated with embryonic developmental competence (r = 0.66, P < 0.1), relative expression of BCL2-L1 (r = 0.72, P < 0.05) and ratio of BCL2-L1/BAK1 (r = 0.78, P < 0.05). In conclusion, our data show that IGF-II, BCL2-L1 and BAK1 expression is not related to the chosen combination of paternal breed, but that IGF-II expression is correlated with embryonic viability and apoptosis-related gene expression.
父本品种对早期胚胎以及后期产前和产后发育的影响已有充分记录。最近的几项研究表明，这种父本效应可能是由早期胚胎发生过程中父本诱导的表观遗传修饰介导的。本研究的目的是调查父本品种对体外生产（IVP）的牛胚胎早期胚胎发育以及母源印记基因IGF-II、凋亡相关基因BAK1和BCL2-L1相对表达的影响，这些胚胎来自两个不相关的父本品种（荷斯坦牛和瑞士褐牛）。还研究了IGF-II表达模式与胚胎发育能力和凋亡相关基因的相关程度。使用比较Cp法通过定量逆转录聚合酶链反应测量第8天胚胎中IGF-II、BCL2-L1和BAK1转录本的相对丰度。我们的数据显示，父本品种不影响第8天囊胚的分裂率、囊胚率以及IGF-II、BAK1和BCL2-L1的相对丰度（P>0.05）。然而，IGF-II表达水平与胚胎发育能力高度相关（r=0.66，P<0.1）、与BCL2-L1的相对表达相关（r=0.72，P<0.05）以及与BCL2-L1/BAK1的比率相关（r=0.78，P<0.05）。总之，我们的数据表明，IGF-II、BCL2-L1和BAK1的表达与所选的父本品种组合无关，但IGF-II的表达与胚胎活力和凋亡相关基因的表达相关。
