少即是多：具有最小约束的困难靶点结构
Less is more: structures of difficult targets with minimal constraints.
作者信息
Lloyd Neil R, Wuttke Deborah S
机构信息
Department of Chemistry and Biochemistry, University of Colorado, 596 UCB, Boulder, CO 80309, USA.
Department of Chemistry and Biochemistry, University of Colorado, 596 UCB, Boulder, CO 80309, USA.
出版信息
Structure. 2014 Sep 2;22(9):1223-1224. doi: 10.1016/j.str.2014.08.004.
Abstract
By merging recent experimental and computational methodology advances, resolution-adapted structural recombination Rosetta has emerged as a powerful strategy for solving the structure of traditionally challenging targets. In this issue of Structure, Sgourakis and colleagues solve the structure of one such target, the immunoevasin protein m04, using this approach.
摘要
通过融合近期实验和计算方法的进展，分辨率自适应结构重组罗塞塔方法已成为解决传统上具有挑战性的目标结构的强大策略。在本期《结构》杂志中，斯古拉基斯及其同事使用这种方法解析了一个这样的目标蛋白——免疫逃逸蛋白m04的结构。
相似文献
1
Less is more: structures of difficult targets with minimal constraints.
Structure. 2014 Sep 2;22(9):1223-1224. doi: 10.1016/j.str.2014.08.004.
2
The structure of the cytomegalovirus-encoded m04 glycoprotein, a prototypical member of the m02 family of immunoevasins.
J Biol Chem. 2014 Aug 22;289(34):23753-63. doi: 10.1074/jbc.M114.584128. Epub 2014 Jun 30.
3
The structure of mouse cytomegalovirus m04 protein obtained from sparse NMR data reveals a conserved fold of the m02-m06 viral immune modulator family.
Structure. 2014 Sep 2;22(9):1263-1273. doi: 10.1016/j.str.2014.05.018. Epub 2014 Aug 7.
4
An endocytic YXXΦ (YRRF) cargo sorting motif in the cytoplasmic tail of murine cytomegalovirus AP2 'adapter adapter' protein m04/gp34 antagonizes virus evasion of natural killer cells.
Med Microbiol Immunol. 2015 Jun;204(3):383-94. doi: 10.1007/s00430-015-0414-1. Epub 2015 Apr 8.
5
Automatically Fixing Errors in Glycoprotein Structures with Rosetta.
Structure. 2019 Jan 2;27(1):134-139.e3. doi: 10.1016/j.str.2018.09.006. Epub 2018 Oct 18.
6
Systematic evaluation of CS-Rosetta for membrane protein structure prediction with sparse NOE restraints.
Proteins. 2017 May;85(5):812-826. doi: 10.1002/prot.25224. Epub 2017 Feb 28.
7
Order out of complexity--protein structures that interact with heparin.
Curr Opin Struct Biol. 2001 Oct;11(5):623-8. doi: 10.1016/s0959-440x(00)00257-8.
8
Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility.
Methods Mol Biol. 2015;1331:93-121. doi: 10.1007/978-1-4939-2874-3_7.
9
Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems.
Methods Mol Biol. 2017;1607:455-466. doi: 10.1007/978-1-4939-7000-1_19.
10
The putative natural killer decoy early gene m04 (gp34) of murine cytomegalovirus encodes an antigenic peptide recognized by protective antiviral CD8 T cells.
J Virol. 2000 Feb;74(4):1871-84. doi: 10.1128/jvi.74.4.1871-1884.2000.
引用本文的文献
1
NMR structure determination of the protein NP_344798.1 as the first representative of Pfam PF06042.
J Biomol NMR. 2015 Jan;61(1):83-7. doi: 10.1007/s10858-014-9878-3. Epub 2014 Nov 28.
2
J-UNIO protocol used for NMR structure determination of the 206-residue protein NP_346487.1 from Streptococcus pneumoniae TIGR4.
J Biomol NMR. 2015 Jan;61(1):65-72. doi: 10.1007/s10858-014-9886-3. Epub 2014 Nov 27.
3
A hybrid NMR/SAXS-based approach for discriminating oligomeric protein interfaces using Rosetta.
Proteins. 2015 Feb;83(2):309-17. doi: 10.1002/prot.24719. Epub 2014 Dec 19.
本文引用的文献
1
The structure of mouse cytomegalovirus m04 protein obtained from sparse NMR data reveals a conserved fold of the m02-m06 viral immune modulator family.
Structure. 2014 Sep 2;22(9):1263-1273. doi: 10.1016/j.str.2014.05.018. Epub 2014 Aug 7.
2
Structure of Est3 reveals a bimodal surface with differential roles in telomere replication.
Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):214-8. doi: 10.1073/pnas.1316453111. Epub 2013 Dec 16.
3
CASP10 results compared to those of previous CASP experiments.
Proteins. 2014 Feb;82 Suppl 2(0 2):164-74. doi: 10.1002/prot.24448. Epub 2013 Dec 17.
4
Determination of solution structures of proteins up to 40 kDa using CS-Rosetta with sparse NMR data from deuterated samples.
Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):10873-8. doi: 10.1073/pnas.1203013109. Epub 2012 Jun 25.
5
Resolution-adapted recombination of structural features significantly improves sampling in restraint-guided structure calculation.
Proteins. 2012 Mar;80(3):884-95. doi: 10.1002/prot.23245.
6
Structure of the BamC two-domain protein obtained by Rosetta with a limited NMR data set.
J Mol Biol. 2011 Aug 5;411(1):83-95. doi: 10.1016/j.jmb.2011.05.022. Epub 2011 May 23.
7
I-TASSER: a unified platform for automated protein structure and function prediction.
Nat Protoc. 2010 Apr;5(4):725-38. doi: 10.1038/nprot.2010.5. Epub 2010 Mar 25.
8
NMR structure determination for larger proteins using backbone-only data.
Science. 2010 Feb 19;327(5968):1014-8. doi: 10.1126/science.1183649. Epub 2010 Feb 4.
9
Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy.
Nat Protoc. 2006;1(2):749-54. doi: 10.1038/nprot.2006.101.
10
A large scale test of computational protein design: folding and stability of nine completely redesigned globular proteins.
J Mol Biol. 2003 Sep 12;332(2):449-60. doi: 10.1016/s0022-2836(03)00888-x.
Zotero 插件