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双极镰孢对环境因素的反应及其对太阳和紫外线辐射的耐受性。

Reaction of Bipolaris euphorbiae to environmental factors and its tolerance to solar and ultraviolet radiation.

机构信息

Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, s/n, Jaboticabal, SP, 14884-900, Brazil.

出版信息

World J Microbiol Biotechnol. 2011 Jun;27(6):1449-56. doi: 10.1007/s11274-010-0597-y. Epub 2010 Nov 4.

Abstract

The determination of the ideal cultivation conditions and the knowledge of solar radiation tolerance are important aspects that must be investigated for the use of Bipolaris euphorbiae Muchovej and Carvalho as a biocontrol agent. The present study was done to assess the growth rate, sporulation and viability of the fungus cultivated under different conditions of the initial pH value of the growth medium, temperature and photoperiod. The tolerance of this fungus' conidia to light from a source simulating solar and ultraviolet radiation was also assessed. B. euphorbiae was affected by different pH values of the growth medium being 6.0 the adequate pH for fungus cultivation. Incubation of the fungus under temperatures of 22, 25 and 28°C enhanced mycelium growth, while sporulation and viability of conidia were better at 22 and 16°C. Different regimens of exposure to light of fungal cultures only affect sporulation. Conidia exposed to solar and ultraviolet radiation for 8 h and 90 min, respectively, presented viabilities higher than 92%, indicating high tolerance levels to radiation exposure.

摘要

确定理想的培养条件和太阳辐射耐受性的知识是将双极镰刀菌(Bipolaris euphorbiae Muchovej 和 Carvalho)用作生物防治剂的重要方面。本研究旨在评估在不同初始培养基 pH 值、温度和光周期条件下培养的真菌的生长率、孢子形成和活力。还评估了该真菌分生孢子对模拟太阳和紫外线辐射光源的光的耐受性。双极镰刀菌受到生长介质不同 pH 值的影响,6.0 是真菌培养的合适 pH 值。在 22、25 和 28°C 的温度下培养真菌会促进菌丝体生长,而在 22 和 16°C 时,分生孢子的孢子形成和活力更好。真菌培养物暴露于不同光照方案仅影响孢子形成。分别暴露于太阳和紫外线辐射 8 小时和 90 分钟的分生孢子的存活率均高于 92%,表明对辐射暴露具有较高的耐受水平。

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