Aita N
Shika Kiso Igakkai Zasshi. 1989 Apr;31(2):184-97. doi: 10.2330/joralbiosci1965.31.184.
The relation between the cytoskeleton and multiplication of cultured endothelial cells was observed immunocytochemically and electron microscopically. Heparin and protamine were used to control the multiplication of the cells. Cytochalasin D, a microfilament synthesis inhibitor, was also used, and its effect on cytoskeletal morphology was compared with the effect of heparin or protamine. In order to quantify changes in microfilaments, the ratio of the length of microfilaments to that of intermediate filaments (M/I ratio) was measured by transmission electron microscopy of the whole mounted cells. The addition of heparin to the culture medium not only enhanced multiplication of the endothelial cells, but also induced the outgrowth of many pseudopodia. In association with these changes, microfilaments became sparse, and stress fibers became short. The addition of protamine to the culture medium suppressed multiplication of endothelial cells and made them spherical. The thickening of the cytoplasmic layer increased the apparent density of the cytoskeleton, but the M/I ratio remained unchanged. Cytochalasin D made the endothelial cells spherical, and at the same time stress fibers disappeared, and microfilaments became sparse. The M/I ratio was smaller than that of the others. Protamine may inactivate protein which forms bridges to connect microfilaments or which binds microfilaments and the cell membrane.
通过免疫细胞化学和电子显微镜观察了细胞骨架与培养的内皮细胞增殖之间的关系。使用肝素和鱼精蛋白来控制细胞的增殖。还使用了微丝合成抑制剂细胞松弛素D，并将其对细胞骨架形态的影响与肝素或鱼精蛋白的影响进行了比较。为了量化微丝的变化，通过对整装细胞进行透射电子显微镜观察，测量微丝长度与中间丝长度的比值（M/I比值）。向培养基中添加肝素不仅增强了内皮细胞的增殖，还诱导了许多伪足的长出。伴随这些变化，微丝变得稀疏，应力纤维变短。向培养基中添加鱼精蛋白抑制了内皮细胞的增殖并使其呈球形。细胞质层的增厚增加了细胞骨架的表观密度，但M/I比值保持不变。细胞松弛素D使内皮细胞呈球形，同时应力纤维消失，微丝变得稀疏。M/I比值比其他情况小。鱼精蛋白可能会使形成连接微丝的桥或结合微丝与细胞膜的蛋白质失活。
