Liu Yu C, Ud-Din Abu I, Roujeinikova Anna
Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia.
Acta Crystallogr F Struct Biol Commun. 2014 Sep;70(Pt 9):1276-9. doi: 10.1107/S2053230X14015398. Epub 2014 Aug 27.
Helicobacter pylori infection is the common cause of gastritis and duodenal and stomach ulcers, which have been linked to a higher risk of the development of gastric cancer. The motility that facilitates persistent infection requires functional flagella that are heavily glycosylated with 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid (pseudaminic acid). Pseudaminic acid biosynthesis protein H (PseH) catalyzes the third step in its biosynthetic pathway, producing UDP-2,4-diacetamido-2,4,6-trideoxy-β-L-altropyranose. Crystals of H. pylori PseH have been grown by the hanging-drop vapour-diffusion method using diammonium tartrate as a precipitating agent. The crystals belonged to space group I222 or I212121, with unit-cell parameters a = 107.8, b = 145.4, c = 166.3 Å. A complete X-ray diffraction data set has been collected to 2.5 Å resolution using cryocooling conditions and synchrotron radiation.
幽门螺杆菌感染是胃炎、十二指肠溃疡和胃溃疡的常见病因，这些疾病与胃癌发生风险较高有关。有助于持续感染的运动性需要功能正常的鞭毛，这些鞭毛高度糖基化，含有5,7-二乙酰氨基-3,5,7,9-四脱氧-L-甘油-L-甘露糖壬酮糖酸（假氨基糖酸）。假氨基糖酸生物合成蛋白H（PseH）催化其生物合成途径的第三步，生成UDP-2,4-二乙酰氨基-2,4,6-三脱氧-β-L-阿卓吡喃糖。幽门螺杆菌PseH的晶体通过悬滴气相扩散法生长，使用酒石酸二铵作为沉淀剂。晶体属于空间群I222或I212121，晶胞参数a = 107.8、b = 145.4、c = 166.3 Å。使用低温冷却条件和同步辐射，已收集到完整的X射线衍射数据集，分辨率达到2.5 Å。
