Sun Meizhou, Jin Wei, Li Yuanfei, Mao Shengyong, Cheng Yanfen, Zhu Weiyun
Wei Sheng Wu Xue Bao. 2014 May 4;54(5):563-71.
We studied the microbial interaction between anaerobic fungi and methanogens in the rumen of Holstein Cow.
Co-cultures of anaerobic fungi with indigenously associated methanogen were isolated by Hungate roll-tube technique. The anaerobic fungi were identified by morphology and 4', 6 diamidino-2-phylindole nucleus staining and the methanogens were identified by 16S rRNA gene sequencing.
A total of 28 co-cultures of anaerobic fungus with indigenously associated methanogen were obtained. The anaerobic fungi in the co-cultures were identified as monocentric genera Piromyces, Neocallimastix and Caeomyces. The indigenously associated methanogens were Methanobrevibacter olleyae like and Methanobrevibacter thaueri like strains. Four different phylotypes of fungus-methanogen co-cultures were obtained, which were Piromyces/Methanobrevibacter olleyae like strains, Neocallimastix/ Methanobrevibacter olleyae like strains, Neocallimastix/Methanobrevibacter thaueri like strains and Caecomyces/ Methanobrevibacter olleyae like strains.
Our study isolated and identified 28 co-cultures of anaerobic fungus and associated methanogens, which provided new materials for further study the mechanism of methane emission in the rumen.
我们研究了荷斯坦奶牛瘤胃中厌氧真菌与产甲烷菌之间的微生物相互作用。
采用Hungate滚管技术分离厌氧真菌与本地相关产甲烷菌的共培养物。通过形态学和4',6-二脒基-2-苯基吲哚核染色鉴定厌氧真菌，通过16S rRNA基因测序鉴定产甲烷菌。
共获得28株厌氧真菌与本地相关产甲烷菌的共培养物。共培养物中的厌氧真菌被鉴定为单中心属的梨形霉属、新美鞭菌属和嗜纤维菌属。本地相关产甲烷菌为类奥氏甲烷短杆菌和类索氏甲烷短杆菌菌株。获得了四种不同的真菌-产甲烷菌共培养物系统发育型，分别是梨形霉属/类奥氏甲烷短杆菌菌株、新美鞭菌属/类奥氏甲烷短杆菌菌株、新美鞭菌属/类索氏甲烷短杆菌菌株和嗜纤维菌属/类奥氏甲烷短杆菌菌株。
我们的研究分离并鉴定了28株厌氧真菌和相关产甲烷菌的共培养物，为进一步研究瘤胃甲烷排放机制提供了新材料。
