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[从黑海西北部多菲尼夫斯基莱曼和敖德萨地区沉积物中分离出的磷素活化细菌]

[Phosphate-mobilizing bacteria isolated from sediments of Dofinivskyi Lyman and Odessa region of north-western part of the Black Sea].

作者信息

Pavlova H H, Roĭ A O, Kurdysh I K

出版信息

Mikrobiol Z. 2014 Jul-Aug;76(4):34-40.

Abstract

Active strains of phosphate-mobilizing bacteria were isolated from sediments of Odessa region of northwestern part of the Black Sea (NWBS) and Dofinivskyi Lyman. Two strains of bacteria were characterized by the highest activity among the selected isolates. Their morphological and physiological-biochemical properties, their genus identity and growth dynamics in a nutrient medium with insoluble calcium phosphate have been investigated. It has been established that the logarithmic phase of bacterial growth began after 6 hours of cultivation. The number of cells after 2 days of cultivation increased from 10(5) - 10(6) to 10(8) - 10(9) CFU cm(-3). This was accompanied by a decrease in pH from 7.1 to 4.56-4.86. The studied bacteria from Odessa region NWBS, identified as Enterobacter sp. 18, accumulated in the culture medium up to 240 mg PO+4(3-)/l and from Dofinivskyi Lyman - Bacillus sp. 5 - 460 mg PO4(3-)/l.

摘要

从黑海西北部敖德萨地区(NWBS)和多菲尼夫斯基莱曼的沉积物中分离出具有活性的解磷细菌菌株。在所挑选出的分离菌株中,有两种菌株表现出最高的活性。研究了它们的形态学、生理生化特性、属的鉴定以及在含有不溶性磷酸钙的营养培养基中的生长动态。已确定细菌生长的对数期在培养6小时后开始。培养2天后,细胞数量从10(5)-10(6)增加到10(8)-10(9) CFU cm(-3)。与此同时,pH值从7.1降至4.56 - 4.86。从敖德萨地区NWBS分离出的经鉴定为肠杆菌属18的研究细菌,在培养基中积累的PO+4(3-)/l高达240毫克,而从多菲尼夫斯基莱曼分离出的芽孢杆菌属5积累的PO4(3-)/l为460毫克。

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