Shiratori Y, Jin'nai H, Teraoka H, Matano S, Matsumoto K, Kamii K, Tanaka M, Okano K
2nd Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Exp Cell Biol. 1989;57(6):300-9. doi: 10.1159/000163542.
The balance of phagocytic function among Kupffer cells, hepatic endothelial cells and splenic macrophages in the chronically ethanol-fed rats has been investigated. Clearance of latex particles in the blood was measured to estimate the function of the reticuloendothelial system. Phagocytosis of latex particles by Kupffer cells, hepatic endothelial cells or splenic macrophages in vivo was measured by counting the number of ingested particles in a cell after isolation of hepatic nonparenchymal cells or spleen cells following injection of different amounts of latex particles. Latex particle clearance was suppressed in the ethanol-fed rats, demonstrating a decreased phagocytic capacity of the reticuloendothelial system. Markedly decreased phagocytic function was found in 40% of Kupffer cells of the chronically ethanol-fed rats. In contrast, the number of latex particles in hepatic endothelial cells and in splenic macrophages was increased after injection of a triggering dose of latex particles. From these results it may be concluded that an increased phagocytosis of hepatic endothelial cells and splenic macrophages could compensate for the decreased phagocytic function of Kupffer cells.
对长期喂食乙醇的大鼠肝巨噬细胞、肝内皮细胞和脾巨噬细胞吞噬功能的平衡进行了研究。通过测量血液中乳胶颗粒的清除率来评估网状内皮系统的功能。在注射不同量的乳胶颗粒后，分离肝非实质细胞或脾细胞，通过计数细胞内摄入颗粒的数量来测量体内肝巨噬细胞、肝内皮细胞或脾巨噬细胞对乳胶颗粒的吞噬作用。喂食乙醇的大鼠中乳胶颗粒清除率受到抑制，表明网状内皮系统的吞噬能力下降。在长期喂食乙醇的大鼠中，40%的肝巨噬细胞吞噬功能明显降低。相反，注射触发剂量的乳胶颗粒后，肝内皮细胞和脾巨噬细胞内的乳胶颗粒数量增加。从这些结果可以得出结论，肝内皮细胞和脾巨噬细胞吞噬作用的增强可以弥补肝巨噬细胞吞噬功能的降低。
