Gao Lin, Wang Jixiang, Li Xiuying, Yan Yongsheng, Li Chunxiang, Pan Jianming
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, China.
Anal Bioanal Chem. 2014 Nov;406(28):7213-20. doi: 10.1007/s00216-014-8126-8. Epub 2014 Sep 9.
In this study, we report here a general protocol for making core-shell magnetic Fe3O4/SiO2-MPS/MIPs (MPS = 3-(methacryloxyl) propyl trimethoxysilane, MIPs = molecularly imprinted polymers, Fe3O4/SiO2-MPS as core, MIPs as shell) via a surface molecular imprinting technique for optical detection of trace λ-cyhalothrin. The fluorescent molecularly imprinted polymer shell was first prepared by copolymerization of acrylamide with a small quantity of allyl fluorescein in the presence of λ-cyhalothrin to form recognition sites without doping. The magnetic Fe3O4/SiO2-MPS/MIPs exhibited paramagnetism, high fluorescence intensity, and highly selective recognition. Using fluorescence quenching as a detecting tool, Fe3O4/SiO2-MPS/MIPs were successfully applied to selectively and sensitively detect λ-cyhalothrin, and a linear relationship could be obtained covering a wide concentration range of 0-50 nM with a correlation coefficient of 0.9962 described by the Stern-Volmer equation. The experimental results of practical detection revealed that magnetic Fe3O4/SiO2-MPS/MIPs as an attractive recognition element was satisfactory for determination of trace λ-cyhalothrin in honey samples. This study, therefore, demonstrated the potential of MIPs for detection of λ-cyhalothrin in food.
在本研究中，我们报道了一种通用的制备核壳型磁性Fe3O4/SiO2-MPS/MIPs（MPS = 3-(甲基丙烯酰氧基)丙基三甲氧基硅烷，MIPs = 分子印迹聚合物，以Fe3O4/SiO2-MPS为核，MIPs为壳）的方法，该方法通过表面分子印迹技术用于痕量氯氟氰菊酯的光学检测。荧光分子印迹聚合物壳首先通过丙烯酰胺与少量烯丙基荧光素在氯氟氰菊酯存在下共聚制备，以形成无掺杂的识别位点。磁性Fe3O4/SiO2-MPS/MIPs表现出顺磁性、高荧光强度和高度选择性识别。以荧光猝灭作为检测工具，Fe3O4/SiO2-MPS/MIPs成功应用于选择性和灵敏地检测氯氟氰菊酯，通过Stern-Volmer方程可获得在0 - 50 nM宽浓度范围内的线性关系，相关系数为0.9962。实际检测的实验结果表明，磁性Fe3O4/SiO2-MPS/MIPs作为一种有吸引力的识别元件，用于测定蜂蜜样品中的痕量氯氟氰菊酯是令人满意的。因此，本研究证明了分子印迹聚合物在食品中氯氟氰菊酯检测方面的潜力。
