School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
School of Material Science and Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
Food Chem. 2014 Aug 1;156:1-6. doi: 10.1016/j.foodchem.2013.12.065. Epub 2013 Dec 25.
In this study, we first present a general protocol for making fluorescent molecularly imprinted polymer microspheres via precipitation polymerisation. We first prepared the fluorescent molecularly imprinted polymer microspheres upon copolymerisation of acrylamide with a small quantity of allyl fluorescein in the presence of cyhalothrin to form recognition sites without doping. The as-synthesised microspheres exhibited spherical shape, high fluorescence intensity and highly selective recognition. Under optical conditions, polymer microspheres were successfully applied to selectively and sensitively detect cyhalothrin, and a linear relationship could be obtained covering the lower concentration range of 0-1.0nM with a correlation coefficient of 0.9936 described by the Stern-Volmer equation. A lower limit of detection was found to be 0.004nM. The results of practical detection suggested that the developed method was satisfactory for determination of cyhalothrin in honey samples. This study therefore demonstrated the potential of molecularly imprinted polymers for detection of cyhalothrin in food.
在这项研究中，我们首先提出了一种通过沉淀聚合制备荧光分子印迹聚合物微球的通用方案。我们首先在存在氯氟氰菊酯的情况下，将丙烯酰胺与少量烯丙基荧光素共聚，形成没有掺杂的识别位点，从而制备出荧光分子印迹聚合物微球。所合成的微球呈现出球形、高荧光强度和高度选择性识别。在光学条件下，聚合物微球成功地应用于选择性和灵敏地检测氯氟氰菊酯，并且可以通过 Stern-Volmer 方程获得涵盖 0-1.0nM 低浓度范围的线性关系，相关系数为 0.9936。检测的下限为 0.004nM。实际检测结果表明，所开发的方法可用于测定蜂蜜样品中的氯氟氰菊酯。因此，本研究证明了分子印迹聚合物在检测食品中氯氟氰菊酯方面的潜力。
