[不同类型跳跃对大鼠胫骨中白细胞介素-6、骨保护素和核因子κB受体活化因子配体表达的影响]

[Effect of different types of jumps on the expressions of IL-6, OPG and RANKL in rat tibia].

作者信息

Wang Ting, Zhang Xinhua, Li Shengcun

机构信息

Institute of Biotechnology, Soochow University, Suzhou 215007; School of Biology and Basic Medical Science, Medical College, Soochow University, Suzhou 215123, China.

School of Physical Education, Soochow University, Suzhou 215021, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 Sep;30(9):909-12.

PMID:25200151

Abstract

OBJECTIVE

To investigate the effect of different types of jumps on the expressions of interleukin 6 (IL-6), osteoprotegerin(OPG), ligand of receptor activator of NF-κB(RANKL) genes related to bone metabolism in rat tibia, and explore the molecular mechanism underlying bone destruction associated with over-loading jumping.

METHODS

Twenty-four male SD rats were randomly assigned to control group (C), jump group (J) and loading jump group (JL), 8 rats in each group. J group participated in incremental load training. The rats in JL group carried loads that were 5% of their body mass. Same training plan was used in J and JL groups, while control group was not treated. The experiment was completed after 6 weeks. The serum samples were collected and assayed for the contents of alkaline phosphatase (ALP) and tartrate resistant acid phosphatase (TRACP). One-side tibia was used for paraffin section and HE staining, and the other side was used for real time quantitative PCR(qRT-PCR) to detect the expressions of IL-6, OPG and RANKL mRNA.

RESULTS

Compared with control group and J group, the collagen fibers in periost was more disordered in JL group. We further observed a more serious damage of the cortical bone and increased cavities in JL group. The serum ALP levels in JL group were significantly higher than those in control and J groups (P<0.05). The serum TRACP levels in JL and J groups were significantly higher compared to control group (P<0.05). Furthermore, there was a significantly higher expression level of IL-6 in both jump groups (P<0.01), while the JL group had a higher IL-6 expression compared with J group (P<0.01). There was a higher expression of OPG mRNA in JL and J groups (P<0.05). The highest expression level of OPG was observed in J group. The expression level of RANKL was significantly higher in JL group compared with control and J groups (P<0.01).

CONCLUSION

After loading jump for 6 weeks, the expressions of IL-6 and RANKL were markedly elevated in rat tibia, suggesting that IL-6 and RANKL might be involved in the process of bone destruction due to increased bone turnover.

摘要

目的

探讨不同类型跳跃运动对大鼠胫骨中与骨代谢相关的白细胞介素6（IL-6）、骨保护素（OPG）、核因子κB受体激活剂配体（RANKL）基因表达的影响，探索与过度负荷跳跃相关的骨破坏的分子机制。

方法

将24只雄性SD大鼠随机分为对照组（C）、跳跃组（J）和负荷跳跃组（JL），每组8只。J组进行递增负荷训练。JL组大鼠背负其体重5%的负荷。J组和JL组采用相同的训练方案，而对照组不进行处理。6周后完成实验。采集血清样本，检测碱性磷酸酶（ALP）和抗酒石酸酸性磷酸酶（TRACP）的含量。一侧胫骨用于石蜡切片和HE染色，另一侧用于实时定量PCR（qRT-PCR）检测IL-6、OPG和RANKL mRNA的表达。

结果

与对照组和J组相比，JL组骨膜中的胶原纤维更加紊乱。我们进一步观察到JL组皮质骨损伤更严重，空洞增加。JL组血清ALP水平显著高于对照组和J组（P<0.05）。JL组和J组血清TRACP水平显著高于对照组（P<0.05）。此外，两个跳跃组中IL-6的表达水平均显著升高（P<0.01），而JL组IL-6的表达高于J组（P<0.01）。JL组和J组OPG mRNA表达较高（P<0.05）。J组OPG表达水平最高。JL组RANKL的表达水平显著高于对照组和J组（P<0.01）。