Tevkin S, Lokshin V, Shishimorova M, Polumiskov V
Institute of Reproductive Medicine, "EKO" Centre , Almaty , Kazakhstan.
Gynecol Endocrinol. 2014 Oct;30 Suppl 1:9-12. doi: 10.3109/09513590.2014.945767.
The application in IVF practice of modern techniques can improve positive outcome of each cycle in the assisted reproductive technology (ART) programs and the effectiveness of treatment as a whole. There are embryos in the female reproductive tract in physiological medium which contain various cytokines and growth factors. It plays an important role in the regulation of normal embryonic development, improve implantation and subsequently optimizing the development of the fetus and the placenta. Granulocyte macrophage colony-stimulating factor (GM-CSF is one of the cytokines playing an important role in reproductive function. Addition of recombinant GM-CSF to the culture medium can makes closer human embryos culture to in vivo conditions and improve the efficacy ART cycles. The analysis of culture embryos in EmbryoGen medium has shown that fertilization rate embryo culture and transfer to patients with previous unsuccessful attempts increases clinical pregnancy rate compared to the control group 39.1 versus 27.8%, respectively. It is noted that the implantation rate (on 7 weeks' gestation) and progressive clinical pregnancy rate (on 12 weeks' gestation) were significantly higher in group embryos culture in EmbryoGen medium compared to standard combination of medium (ISM1+VA), and were 20.4 and 17.4% versus 11.6 and 9.1%, respectively.
现代技术在体外受精实践中的应用可以提高辅助生殖技术（ART）项目中每个周期的阳性结果以及整体治疗效果。在生理介质中，女性生殖道内存在含有各种细胞因子和生长因子的胚胎。它在正常胚胎发育的调节、改善着床以及随后优化胎儿和胎盘的发育中起着重要作用。粒细胞巨噬细胞集落刺激因子（GM-CSF）是在生殖功能中起重要作用的细胞因子之一。向培养基中添加重组GM-CSF可使人类胚胎培养更接近体内条件，并提高ART周期的疗效。对在EmbryoGen培养基中培养的胚胎的分析表明，与对照组相比，将在EmbryoGen培养基中培养并移植给先前尝试未成功的患者的胚胎的受精率、胚胎培养和移植可提高临床妊娠率，分别为39.1%和27.8%。值得注意的是，与标准培养基组合（ISM1+VA）相比，在EmbryoGen培养基中培养的胚胎组的着床率（妊娠7周时）和进行性临床妊娠率（妊娠12周时）显著更高，分别为20.4%和17.4%，而标准组合组分别为11.6%和9.1%。
