Yourek Gregory, Xin Xuejun, Reilly Gwendolen C, Mao Jeremy J
DL Biotech USA, Gaithersburg, MD, USA.
Theracell, Northridge, CA, USA.
Biomed Mater Eng. 2014;24(5):1803-15. doi: 10.3233/BME-140991.
Attachment of cells to fully hydrated hydrogel biomaterials, such as PEGDA, has proven challenging because of the hydrophobic cellular membrane.
To demonstrate undifferentiated human mesenchymal stem cells (hMSCs) and hMSC-derived chondrocytes infiltrated and attached to unmodified PEGDA hydrogel.
Human MSCs and MSC-Cys were cultured in and on PEGDA hydrogel. Attachment was verified by SEM and confocal microscopy and was accompanied by vinculin expression, indicating the presence of focal contacts. Infiltration was confirmed by H&E and fluorescence staining.
Cells cultured on top of PEGDA hydrogel infiltrated the material on the order of micrometers.
These findings will aid in understanding the cell-scaffold interaction for regenerative medicine constructs.
由于细胞膜具有疏水性，细胞附着于完全水合的水凝胶生物材料（如聚乙二醇二丙烯酸酯，PEGDA）已被证明具有挑战性。
证明未分化的人间充质干细胞（hMSCs）和hMSC来源的软骨细胞能够浸润并附着于未修饰的PEGDA水凝胶。
将人骨髓间充质干细胞和MSC-Cys在PEGDA水凝胶中及表面进行培养。通过扫描电子显微镜（SEM）和共聚焦显微镜验证细胞附着情况，并观察纽蛋白表达，以表明焦点黏附的存在。通过苏木精-伊红（H&E）染色和荧光染色确认细胞浸润情况。
在PEGDA水凝胶表面培养的细胞可浸润至材料内达微米级深度。
这些发现将有助于理解再生医学构建物中细胞与支架的相互作用。
