Maurya Dharmendra Kumar
Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra, India
Int J Toxicol. 2014 Sep-Oct;33(5):362-6. doi: 10.1177/1091581814549961. Epub 2014 Sep 7.
Although Halo assay is a fast and more economic technique, it is not popular compared to comet assay for the measurement of DNA damage. One of the reasons behind this was nonavailability of suitable user-friendly program. Currently, most of the researchers were analyzing halo images manually using image analysis software (Scion Image or ImageJ). To address this problem, I have developed a semiautomatic halo analysis ImageJ program, HaloJ, and applied in the assessment of DNA damage at the single-cell level. In this article, we have shown that data obtained from the HaloJ program have a very good correlation with the data obtained using comet assay analysis program such as Comet Assay Software Project. To the best of our knowledge, this will be the first program to quantify DNA damage of halo images. This program will be of great use for researchers working on the DNA damage and repair, radiation biology, toxicology, cancer biology, and so on.
尽管光晕试验是一种快速且更经济的技术，但与彗星试验相比，它在测量DNA损伤方面并不受欢迎。造成这种情况的原因之一是缺乏合适的用户友好型程序。目前，大多数研究人员使用图像分析软件（Scion Image或ImageJ）手动分析光晕图像。为了解决这个问题，我开发了一个半自动光晕分析ImageJ程序HaloJ，并将其应用于单细胞水平的DNA损伤评估。在本文中，我们已经表明，从HaloJ程序获得的数据与使用彗星试验分析程序（如彗星试验软件项目）获得的数据具有非常好的相关性。据我们所知，这将是第一个量化光晕图像DNA损伤的程序。该程序对于从事DNA损伤与修复、辐射生物学、毒理学、癌症生物学等研究的人员将非常有用。
