Buelach Hospital, Buelach, Switzerland.
Department of Gynaecology and Obstetrics, Maennedorf Hospital, Maennedorf, Switzerland.
Anticancer Res. 2014 Sep;34(9):4693-9.
Distinct populations of neutrophils have been identified based on the expression of intercellular adhesion molecule 1 (ICAM1, CD54) and chemokine receptor 1 (CXCR1, interleukin 8 receptor α).
We analyzed the expression of vascular endothelial growth factor receptor 1 (VEGFR1), a physiological negative regulator of angiogenesis, on distinct populations of neutrophils from the blood of patients before and after adjuvant chemotherapy for breast cancer.
Neutrophil populations were distinguished as reverse transmigrated (ICAM1(high)/CXCR1(low)), naïve (ICAM1(low)/CXCR1(high)), or tissue-resident neutrophils (ICAM1(low)/CXCR1(low)), and their VEGFR1 expression quantified.
Reverse transmigrated ICAM1(high)/CXCR1(low) neutrophilic granulocytes decreased significantly after chemotherapy and these were also the cells with highest mean fluorescence intensity for VEGFR1.
Chemotherapy mainly reduces the number of reverse transmigrated long-lived ICAM1(high)/CXCR1(low) VEGFR1-expressing neutrophils. The decrease of antiangiogenic VEGFR1 may have a potential impact on tumour angiogenesis in patients undergoing adjuvant chemotherapy.
根据细胞间黏附分子 1（ICAM1，CD54）和趋化因子受体 1（CXCR1，白细胞介素 8 受体 α）的表达，已经鉴定出不同群体的中性粒细胞。
我们分析了血管内皮生长因子受体 1（VEGFR1）在乳腺癌辅助化疗前后患者血液中不同中性粒细胞群体中的表达。VEGFR1 是血管生成的生理负调节剂。
将中性粒细胞群体区分成反向迁移（ICAM1（高）/CXCR1（低））、幼稚（ICAM1（低）/CXCR1（高））或组织驻留中性粒细胞（ICAM1（低）/CXCR1（低）），并定量其 VEGFR1 表达。
化疗后，反向迁移的 ICAM1（高）/CXCR1（低）中性粒细胞显著减少，这些细胞的 VEGFR1 平均荧光强度也最高。
化疗主要减少抗血管生成 VEGFR1 表达的反向迁移长寿命 ICAM1（高）/CXCR1（低）中性粒细胞数量。接受辅助化疗的患者中，抗血管生成 VEGFR1 的减少可能对肿瘤血管生成产生潜在影响。
