载药杂合脂质体纳米疗法用于结直肠癌的体外与体内凋亡研究
Nanotherapy with hybrid liposomes for colorectal cancer along with apoptosis in vitro and in vivo.
机构信息
Division of Applied Life Science, Graduate School of Engineering, Sojo University, Kumamoto, Japan.
Immuno-medical Lab, Omote Sando Yoshida Hospital, Kumamoto, Japan.
出版信息
Anticancer Res. 2014 Sep;34(9):4701-8.
AIM
We examined the therapeutic effects of hybrid liposomes (HL) composed of L-α-dimyristylphosphati-dylcholine (DMPC) and polyoxyethylene (25) dodecyl ether (C12(EO)25) on the growth of human colorectal cancer (WiDr) cells in vitro and in vivo.
MATERIALS AND METHODS
HL composed of 95 mol% DMPC and 5 mol% C12(EO)25 were prepared by the sonication method and their therapeutic effects in xenograft mouse models of colorectal cancer liver metastases were examined in vivo.
RESULTS
The inhibitory effects of HL-25 on the growth of WiDr cells along with apoptosis were assessed in vitro. Remarkable inhibitory effects of HL-25 for the liver metastasis of colorectal cancer cells along with apoptosis were revealed on the basis of histological analysis. Prolonged survival was attained for the xenograft mouse model of colorectal cancer after treatment with HL-25 in vivo.
CONCLUSION
Therapeutic effects of HL-25 without any drugs on the liver metastasis of human colorectal cancer were obtained for the first time in vivo.
目的
我们研究了由 L-α-二月桂酰磷脂酰胆碱（DMPC）和聚氧乙烯（25）十二醚（C12（EO）25）组成的杂交脂质体（HL）对体外和体内人结直肠癌细胞（WiDr）生长的治疗作用。
材料和方法
采用超声法制备由 95mol%DMPC 和 5mol%C12（EO）25 组成的 HL，并在结直肠癌肝转移的异种移植小鼠模型中进行体内治疗效果研究。
结果
体外评估了 HL-25 对 WiDr 细胞生长及凋亡的抑制作用。组织学分析显示，HL-25 对结直肠癌细胞肝转移具有显著的抑制作用并诱导凋亡。体内用 HL-25 治疗后，结直肠癌异种移植小鼠模型的生存期延长。
结论
首次在体内获得了 HL-25 对人结直肠癌肝转移无任何药物治疗的疗效。
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