Department of Radiation Oncology, Chonnam National University Medical School, Gwangju, Republic of Korea.
Department of Pathology, Chonnam National University Medical School, Gwangju, Republic of Korea
Anticancer Res. 2014 Sep;34(9):4939-48.
To identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation.
Lung cancer cell lines (A549, NCI-H1650) were irradiated with several fractionation schemes. Clonogenic assays were used to identify radioresistant cell lines. We compared the gene expression profiles on a cDNA microarray.
Four established cell (A549-2G, A549-5G, H1650-2G and H1650-5G) were shown to be radioresistant (p≤0.05). Seventy-two genes were commonly altered in A549-G and 655 genes in H1650-G, compared to their parental cells. Genes in the wingless-type MMTV integration site family (WNT) signaling pathway were the ones most frequently altered in both A549-G and H1650-G cells. Those involved in inflammation; integrin, platelet-derived growth factor (PDGF), interleukin, transforming growth factor-beta (TGFB), epidermal growth factor receptor (EGFR) signaling, were commonly altered in radioresistant H1650 sublines.
The major gene expression changes during irradiation are related to WNT signaling pathway.
为了鉴定经分次照射建立的亲本和耐辐射肺癌细胞系之间差异表达的基因。
用几种分割照射方案照射肺癌细胞系（A549、NCI-H1650）。克隆形成测定法用于鉴定耐辐射细胞系。我们比较了 cDNA 微阵列上的基因表达谱。
四个已建立的细胞系（A549-2G、A549-5G、H1650-2G 和 H1650-5G）表现出耐辐射性（p≤0.05）。与亲本细胞相比，A549-G 中有 72 个基因发生了普遍改变，H1650-G 中有 655 个基因发生了改变。在 A549-G 和 H1650-G 细胞中最常改变的是 Wnt 信号通路中的翼状螺旋转录整合位点家族（WNT）基因。那些涉及炎症、整合素、血小板衍生生长因子（PDGF）、白细胞介素、转化生长因子-β（TGFB）、表皮生长因子受体（EGFR）信号的基因在耐辐射的 H1650 亚系中普遍改变。
照射过程中的主要基因表达变化与 WNT 信号通路有关。
