Department of Plant, Soil and Microbial Sciences, Michigan State University, 107 CIPS, East Lansing, Michigan 48824 USA.
Department of Plant Sciences, North Dakota State University, 370G Loftsgard Hall, Fargo, North Dakota 58108 USA.
Appl Plant Sci. 2013 Jan 2;1(1). doi: 10.3732/apps.1200249. eCollection 2013 Jan.
Simple sequence repeat markers were developed based on expressed sequence tags (EST-SSR) and screened for polymorphism among 23 Pisum sativum individuals to assist development and refinement of pea linkage maps. In particular, the SSR markers were developed to assist in mapping of white mold disease resistance quantitative trait loci. •
Primer pairs were designed for 46 SSRs identified in EST contiguous sequences assembled from a 454 pyrosequenced transcriptome of the pea cultivar, 'LIFTER'. Thirty-seven SSR markers amplified PCR products, of which 11 (30%) SSR markers produced polymorphism in 23 individuals, including parents of recombinant inbred lines, with two to four alleles. The observed and expected heterozygosities ranged from 0 to 0.43 and from 0.31 to 0.83, respectively. •
These EST-SSR markers for pea will be useful for refinement of pea linkage maps, and will likely be useful for comparative mapping of pea and as tools for marker-based pea breeding.
基于表达序列标签 (EST-SSR) 开发了简单序列重复标记,并对 23 个豌豆个体进行了多态性筛选,以协助豌豆连锁图谱的开发和细化。特别是,SSR 标记是为了协助绘制白霉病抗性数量性状基因座而开发的。
为从豌豆品种“LIFTER”的 454 焦磷酸测序转录组组装的 EST 连续序列中鉴定的 46 个 SSR 设计了引物对。37 对 SSR 标记扩增了 PCR 产物,其中 11 对 (30%) SSR 标记在 23 个个体中产生了多态性,包括重组自交系的亲本,具有 2 到 4 个等位基因。观察到的和预期的杂合度分别在 0 到 0.43 和 0.31 到 0.83 之间。
这些用于豌豆的 EST-SSR 标记将有助于细化豌豆连锁图谱,并可能有助于豌豆的比较作图以及作为基于标记的豌豆育种工具。