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豌豆（豆科）中新型 EST 衍生基因标记的分离与鉴定。

Isolation and characterization of novel EST-derived genic markers in Pisum sativum (Fabaceae).

机构信息

Department of Plant Sciences, North Dakota State University Fargo, North Dakota 58108-6050 USA.

出版信息

Appl Plant Sci. 2013 Oct 28;1(11). doi: 10.3732/apps.1300026. eCollection 2013 Nov.

DOI:10.3732/apps.1300026
PMID:25202494
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4103456/
Abstract

PREMISE OF THE STUDY

Novel markers were developed for pea (Pisum sativum) from pea expressed sequence tags (ESTs) having significant homology to Medicago truncatula gene sequences to investigate genetic diversity, linkage mapping, and cross-species transferability. •

METHODS AND RESULTS

Seventy-seven EST-derived genic markers were developed through comparative mapping between M. truncatula and P. sativum in which 75 markers produced PCR products and 33 were polymorphic among 16 pea genotypes. •

CONCLUSIONS

The novel markers described here will be useful for future genetic studies of P. sativum; their amplification in lentil (Lens culinaris) demonstrates their potential for use in closely related species.

摘要

研究前提

从与紫花苜蓿基因序列具有显著同源性的豌豆表达序列标签 (EST) 中开发了新的豌豆标记物，以研究遗传多样性、连锁图谱构建和跨物种可转移性。

方法和结果

通过紫花苜蓿和豌豆之间的比较作图，开发了 77 个 EST 衍生的基因标记物，其中 75 个标记物产生了 PCR 产物，33 个标记物在 16 个豌豆基因型中具有多态性。

结论

本文描述的新标记物将有助于未来豌豆的遗传研究；它们在兵豆（Lens culinaris）中的扩增表明它们在亲缘关系较近的物种中具有应用潜力。

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