首次鉴定勃艮第块菌（块菌科）中的多态微卫星标记。
First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae).
机构信息
UMR Agroécologie INRA 1347/AgroSup/Université de Bourgogne, Pôle Interactions Plantes Microorganismes ERL 6300 CNRS, BP 86510, 21065 Dijon CEDEX, France.
UMR "Interaction Arbres Microorganismes", Centre INRA Nancy, 54280 Champenoux, France.
出版信息
Appl Plant Sci. 2013 Jan 31;1(2). doi: 10.3732/apps.1200220. eCollection 2013 Feb.
PREMISE OF THE STUDY
Tuber aestivum, the most common truffle in Europe, plays an important role in the commercial truffle market. For the first time, microsatellite primers were developed to investigate polymorphism within this species. •
METHODS AND RESULTS
Using direct shotgun pyrosequencing, 15 polymorphic microsatellites were identified out of the 7784 perfect microsatellites present in the 534620 reads obtained. Tested on 75 samples, these microsatellites were highly polymorphic. The number of alleles varied from four to 15, and the expected heterozygosity ranged from 0.266 to 0.620. A multilocus analysis allowed the identification of 63 genotypes over the 75 samples analyzed. •
CONCLUSIONS
Direct shotgun pyrosequencing is a fast and relatively low-cost technique allowing identification of microsatellites in nonmodel species. The microsatellites developed in this study will be useful in population genetic studies to infer the evolutionary history of this species.
研究前提
欧洲最常见的块菌——块菌，在商业块菌市场中发挥着重要作用。本文首次开发了微卫星引物来研究该物种内的多态性。
方法与结果
利用直接焦磷酸测序法，从获得的 7784 个完美微卫星中，发现了 15 个多态性微卫星。在 75 个样本中进行的测试表明，这些微卫星具有高度多态性。等位基因数从 4 到 15 不等，预期杂合度范围从 0.266 到 0.620。通过多点分析，在分析的 75 个样本中确定了 63 种基因型。
结论
直接焦磷酸测序是一种快速且相对低成本的技术，可用于鉴定非模式物种中的微卫星。本研究开发的微卫星将有助于对该物种进化历史进行种群遗传研究。
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