米氏卫星引物在龙舌兰属（天门冬科）中的应用，龙舌兰属是莫哈韦沙漠和科罗拉多高原的关键物种。
Microsatellite primers in Agave utahensis (Asparagaceae), a keystone species in the Mojave Desert and Colorado Plateau.
机构信息
Department of Plant and Wildlife Sciences, Brigham Young University, 4105 Life Sciences Building, Provo, Utah 84602 USA.
出版信息
Appl Plant Sci. 2014 Sep 11;2(9). doi: 10.3732/apps.1400047. eCollection 2014 Sep.
PREMISE OF THE STUDY
Utah agave (Agave utahensis) and its putative subspecies, A. utahensis subsp. kaibabensis and A. utahensis subsp. utahensis, are keystone species of the Mojave Desert and Colorado Plateau in the southwestern United States. Here we developed microsatellite markers to study population structure and genetic diversity of the two subspecies of A. utahensis. •
METHODS AND RESULTS
We analyzed 22,386 454-pyrosequencing large contigs (>400 bp), derived from a genome reduction experiment consisting of A. utahensis accessions, for putative microsatellites. The use of unique multiplex barcodes for each of the Agave accessions allowed for the identification of putatively polymorphic microsatellites based solely on sequence alignment analysis. We report the characteristics of 11 polymorphic microsatellite loci based on a panel of 104 individuals from the two subspecies. The number of alleles per locus varied from three to eight, with an average of 5.5 alleles per locus. Observed and expected heterozygosity values ranged from 0.038 to 0.777 and 0.038 to 0.707, respectively. •
CONCLUSIONS
The microsatellites identified here will be invaluable for future studies of population structure, polyploidy, and genetic diversity across the species.
研究前提
美国犹他龙舌兰（Agave utahensis）及其假定的亚种，即 A. utahensis subsp. kaibabensis 和 A. utahensis subsp. utahensis，是美国西南部莫哈韦沙漠和科罗拉多高原的关键物种。在这里，我们开发了微卫星标记来研究这两个亚种的种群结构和遗传多样性。
方法和结果
我们分析了 22386454 个来自于基因组减少实验的 pyrosequencing 大片段（>400bp），该实验由犹他龙舌兰的样本组成，用于寻找可能的微卫星。每个龙舌兰样本使用独特的多重条形码，仅通过序列比对分析即可识别出可能的多态性微卫星。我们报告了 11 个多态性微卫星位点的特征，这些位点基于来自两个亚种的 104 个个体的面板。每个位点的等位基因数从 3 到 8 不等，平均每个位点有 5.5 个等位基因。观测和预期的杂合度值分别在 0.038 到 0.777 和 0.038 到 0.707 之间。
结论
这里鉴定的微卫星将对未来研究该物种的种群结构、多倍体和遗传多样性具有重要价值。
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