Nielsen E H, Braun K, Johansen T
Institute of Anatomy and Cytology, Odense University, Denmark.
Histol Histopathol. 1989 Oct;4(4):473-7.
The subplasmalemmal cytoskeleton in mast cells has been studied by scanning electron microscopy of the internal side of the plasma membrane. Rearrangement of the dense subplasmalemmal network of actin filaments took place following cell activation by compound 48/80 and secretion of histamine. The rearrangement was a withdrawal of the subplasmalemmal cytoskeleton from the exocytotic sites and the development of bare, filament-free areas around the sites. In calcium-depleted mast cells we demonstrated a dense network that was difficult to break. Activation of the calcium-depleted cells by compound 48/80 did not induce rearrangement of the network, and in parallel there was no secretion of histamine.
通过对质膜内侧进行扫描电子显微镜观察，研究了肥大细胞中的亚质膜细胞骨架。在用化合物48/80激活细胞并分泌组胺后，肌动蛋白丝的致密亚质膜网络发生了重排。这种重排是亚质膜细胞骨架从胞吐部位撤离，以及在这些部位周围形成无细丝的裸露区域。在缺钙的肥大细胞中，我们观察到一个难以破坏的致密网络。用化合物48/80激活缺钙细胞不会诱导网络重排，同时也没有组胺分泌。
