Department of Neurosurgery, Nanjing Children's Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.
Department of Newborn Medicine, Nanjing Children's Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.
Neural Regen Res. 2013 Aug 5;8(22):2078-85. doi: 10.3969/j.issn.1673-5374.2013.22.007.
Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were separated and cultured using the "pour-off" method. Non-adherent bone marrow cell-derived mesenchymal stem cells developed colony-forming unit-fibroblasts, and could be expanded by supplementation with epidermal growth factor. Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor/epidermal growth factor/nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro. Non-adherent bone marrow cell-derived mesenchymal stem cells from β-galactosidase transgenic mice were also transplanted into focal ischemic brain (right corpus striatum) of C57BL/6J mice. At 8 weeks, cells positive for LacZ and β-galactosidase staining were observed in the ischemic tissues, and cells co-labeled with both β-galactosidase and NeuN were seen by double immunohistochemical staining. These findings suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.
从 C57BL/6J 小鼠分离和培养非贴壁骨髓细胞来源的间充质干细胞，采用“倒出”法。非贴壁骨髓细胞来源的间充质干细胞形成集落形成单位纤维母细胞，并可通过补充表皮生长因子进行扩增。免疫细胞化学显示，暴露于碱性成纤维细胞生长因子/表皮生长因子/神经生长因子的非贴壁骨髓细胞来源的间充质干细胞在体外表达神经元特异性标志物神经丝-200 和 NeuN。从β-半乳糖苷酶转基因小鼠分离的非贴壁骨髓细胞来源的间充质干细胞也被移植到 C57BL/6J 小鼠的局灶性缺血性脑（右侧纹状体）中。在 8 周时，在缺血组织中观察到 LacZ 和β-半乳糖苷酶染色阳性的细胞，并通过双重免疫组织化学染色观察到同时标记β-半乳糖苷酶和 NeuN 的细胞。这些发现表明，非贴壁骨髓细胞来源的间充质干细胞可以在体外和体内分化为类神经元细胞。
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