School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, Guangdong Province, China ; Department of Gynecology and Obstetrics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, Guangdong Province, China.
School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
Neural Regen Res. 2013 Jan 25;8(3):258-63. doi: 10.3969/j.issn.1673-5374.2013.03.008.
The sonic hedgehog protein not only plays a key role in early embryonic development, but also has essential effects on the adult nervous system, including neural stem cell proliferation, differentiation, migration and neuronal axon guidance. The N-terminal fragment of sonic hedgehog is the key functional element in this process. Therefore, this study aimed to clone and analyze the N-terminal fragment of the sonic hedgehog gene. Total RNA was extracted from the notochord of a Sprague-Dawley rat at embryonic day 9 and the N-terminal fragment of sonic hedgehog was amplified by nested reverse transcription-PCR. The N-terminal fragment of the sonic hedgehog gene was successfully cloned. The secondary and tertiary structures of the N-terminal fragment of the sonic hedgehog protein were predicted using Jpred and Phyre online.
sonic hedgehog 蛋白不仅在早期胚胎发育中起关键作用，而且对成年神经系统有重要影响，包括神经干细胞增殖、分化、迁移和神经元轴突导向。 sonic hedgehog 的 N 端片段是这一过程中的关键功能元件。因此，本研究旨在克隆和分析 sonic hedgehog 基因的 N 端片段。从胚胎第 9 天的 SD 大鼠脊索中提取总 RNA，通过嵌套反转录-PCR 扩增 sonic hedgehog 的 N 端片段。成功克隆 sonic hedgehog 基因的 N 端片段。使用 Jpred 和 Phyre 在线预测 sonic hedgehog 蛋白 N 端片段的二级和三级结构。
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