Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong Province, China.
Neural Regen Res. 2014 Feb 1;9(3):308-17. doi: 10.4103/1673-5374.128229.
Nestin(+) neurons have been shown to express choline acetyltransferase (ChAT) in the medial septum-diagonal band of Broca in adult rats. This study explored the projection of nestin(+) neurons to the olfactory bulb and the time course of nestin(+) neurons in the medial septum-diagonal band of Broca in adult rats during injury recovery after olfactory nerve transection. This study observed that all nestin(+) neurons were double-labeled with ChAT in the medial septum-diagonal band of Broca. Approximately 53.6% of nestin(+) neurons were projected to the olfactory bulb and co-labeled with fast blue. A large number of nestin(+) neurons were not present in each region of the medial septum-diagonal band of Broca. Nestin(+) neurons in the medial septum and vertical limb of the diagonal band of Broca showed obvious compensatory function. The number of nestin(+) neurons decreased to a minimum later than nestin(-)/ChAT(+) neurons in the medial septum-diagonal band of Broca. The results suggest that nestin(+) cholinergic neurons may have a closer connection to olfactory bulb neurons. Nestin(+) cholinergic neurons may have a stronger tolerance to injury than Nestin(-)/ChAT(+) neurons. The difference between nestin(+) and nestin(-)/ChAT(+) neurons during the recovery process requires further investigations.
巢蛋白（+）神经元在成年大鼠的中隔-斜角带脑区表达胆碱乙酰转移酶（ChAT）。本研究探讨了巢蛋白（+）神经元向嗅球的投射以及嗅神经切断后成年大鼠中隔-斜角带脑区巢蛋白（+）神经元的时间进程。本研究观察到中隔-斜角带脑区的所有巢蛋白（+）神经元均与 ChAT 双标。约 53.6%的巢蛋白（+）神经元投射到嗅球并与快蓝共标。中隔-斜角带脑区的各个区域并没有大量的巢蛋白（+）神经元存在。中隔和斜角带脑区的垂直支的巢蛋白（+）神经元表现出明显的代偿功能。中隔-斜角带脑区的巢蛋白（+）神经元数量的减少晚于巢蛋白（-）/ChAT（+）神经元。结果表明，巢蛋白（+）胆碱能神经元可能与嗅球神经元有更密切的联系。巢蛋白（+）胆碱能神经元对损伤的耐受性可能比巢蛋白（-）/ChAT（+）神经元更强。在恢复过程中，巢蛋白（+）和巢蛋白（-）/ChAT（+）神经元之间的差异需要进一步研究。
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